Enhanced photodynamic therapy using light fractionation against Streptococcus mutans biofilm: type I and type II mechanism

Future Microbiology
Lama Misba, Asad U Khan

Abstract

The objective of the study was to look the efficacy of fractionated light against Streptococcus mutans biofilm. Antibiofilm assays (crystal violet, congo red), electron microscopic, confocal and spectroscopic studies were performed to check the effect of fractionated light. 6-6.5 log10 reduction of planktonic and 3.6-4.2 log10 reduction in biofilm were observed after irradiation with fractionated as compared with continuous light. Increased permeability to propidium iodide and leakage of cellular constituent validate the greater antibiofilm effect of fractionated light. Spectroscopic studies confirmed the relative contribution of type I and type II photochemistry. Phenothiazinium dyes have a potential against bacterial biofilm in combination with light fractionation and it offers new opportunities to explore its clinical implication.

References

Apr 23, 2002·The British Journal of Dermatology·B ZeinaW M Purcell
Feb 4, 2003·Nature Reviews. Drug Discovery·David Davies
Jun 1, 2006·The Journal of Antimicrobial Chemotherapy·Daniel MetcalfSimon Wood
May 8, 2008·Nature Reviews. Microbiology·Mark Achtman, Michael Wagner
Jul 14, 2010·Antimicrobial Agents and Chemotherapy·Liyi HuangMichael R Hamblin
Jul 20, 2010·Free Radical Biology & Medicine·Xavier RagàsSanti Nonell
Apr 13, 2011·International Journal of Oral Science·Niels HøibyThomas Bjarnsholt
Nov 11, 2011·Journal of Photochemistry and Photobiology. B, Biology·Juliana P M L RolimLidiany K A Rodrigues
Apr 14, 2012·Scandinavian Journal of Infectious Diseases·Asad U Khan, Patrice Nordmann
Oct 31, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Henriëtte S de BruijnDominic J Robinson
Feb 9, 2013·Microbiology·José A LemosJacqueline Abranches
Oct 23, 2013·PLoS Pathogens·Khalid H MetwalliMary Ann Jabra-Rizk
Dec 26, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jie ChenPeter M Rentzepis
Apr 1, 2014·Current Opinion in Biotechnology·Seth Yang-En TanLiang Yang
Apr 23, 2014·Photodiagnosis and Photodynamic Therapy·Jing QiaoHuimei Jia
Sep 2, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Kamola R KasimovaMichael R Hamblin
Mar 13, 2015·Frontiers in Cellular and Infection Microbiology·Marlise I KleinHyun Koo
Nov 28, 2015·Applied Microbiology and Biotechnology·Shatavari KulshresthaAsad U Khan
Mar 14, 2016·Journal of Photochemistry and Photobiology. B, Biology·Maria Rita RonquiCarla Raquel Fontana
Aug 31, 2016·British Journal of Cancer·Ana Luiza Ribeiro de SouzaBrian W Pogue

❮ Previous
Next ❯

Citations

May 28, 2019·Antimicrobial Resistance and Infection Control·Divakar SharmaAsad U Khan
Feb 29, 2020·Photochemistry and Photobiology·Rosane Bassi SoaresSilvia Cristina Nunez
Dec 15, 2019·Dentistry Journal·Grace Gomez Felix GomezRichard L Gregory
Mar 17, 2020·Photodiagnosis and Photodynamic Therapy·Leonardo Lobo Ribeiro CavalcanteSilmara Aparecida Milori Corona
Feb 8, 2021·Experimental Eye Research·Konduri RanjithSisinthy Shivaji
Mar 30, 2021·Toxicology·Byanca Thais Lima de SouzaRodrigo Polimeni Constantin
Jun 1, 2021·Frontiers in Medicine·Aleksandra Rapacka-ZdończykJoanna Nakonieczna

❮ Previous
Next ❯

Methods Mentioned

BETA
environmental stress
light fractionation
irradiate
fluorescence spectroscopy
Fluorescence
electrophoresis
PCR
scanning electron microscopy

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.