Enhanced podocalyxin expression alters the structure of podocyte basal surface

Journal of Cell Science
Constantinos G EconomouEffie C Tsilibary

Abstract

Glomerular basement membrane (GBM) and podocalyxin are essential for podocyte morphology. We provide evidence of functional interconnections between basement membrane components (collagen IV and laminin), the expression of podocalyxin and the morphology of human glomerular epithelial cells (podocytes). We demonstrated that GBM and laminin, but not collagen IV, up-regulated the expression of podocalyxin. Scanning electron microscopy revealed that laminin induced a modified morphology of podocytes with process formation, which was more extensive in the presence of GBM. Under high magnification, podocytes appeared ruffled. Using transmission electron microscopy we observed that raised areas occurred in the basal cell surface. Furthermore, the presence of anti-podocalyxin antibody increased the extent of adhesion and spreading of podocytes to both collagen IV and laminin, thus podocalyxin apparently inhibits cell-matrix interactions. We also performed adhesion and spreading assays on podocytes grown under increased glucose concentration (25 mM). Under these conditions, the expression of podocalyxin was almost totally suppressed. The cells adhered and spread to basement membrane components but there was no increase in the extent of ...Continue Reading

References

Sep 1, 1992·Trends in Biochemical Sciences·J HilkensS V Litvinov
Aug 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M T VelasquezO E Michaelis
Nov 1, 1986·The Journal of Clinical Investigation·D KerjaschkiR C Atkins
Apr 10, 1984·Biochemistry·P D Yurchenco, H Furthmayr
Dec 16, 1993·Nature·Y Shimizu, S Shaw
Nov 1, 1994·Kidney International·S S AndersonE C Tsilibary
Nov 1, 1995·Anatomy and Embryology·P Mundel, W Kriz
Nov 1, 1996·Kidney International·C E Kashtan, A F Michael
Dec 1, 1996·The Journal of Clinical Investigation·Y S KanwarE I Wallner
Jan 15, 1997·The Journal of Clinical Investigation·M E PagtalunanT W Meyer
Nov 5, 1997·The Journal of Biological Chemistry·U KrishnamurtiE C Tsilibary
Mar 18, 1999·The American Journal of Pathology·A MiettinenH Holthöfer
Dec 14, 1999·Kidney International·J H Miner
Mar 18, 2000·The Journal of Biological Chemistry·C SassettiS D Rosen
Mar 31, 2000·Nature Genetics·S Somlo, P Mundel
Mar 29, 2001·Journal of the American Society of Nephrology : JASN·Uma KrishnamurtiAlfred Michael
Jul 4, 2001·The Journal of Experimental Medicine·R DoyonnasK M McNagny
Jul 19, 2001·Annals of the New York Academy of Sciences·P J SimmonsD N Haylock
Sep 14, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·P AaltonenH Holthöfer
May 16, 2002·Microscopy Research and Technique·Isao Shirato
Jun 18, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Pauliina LuimulaHarry Holthöfer
Aug 15, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Panagiotis M KaramessinisEffie C Tsilibary
Oct 19, 2002·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Hiroshi KawachiFujio Shimizu
Mar 7, 2003·American Journal of Physiology. Renal Physiology·Paraskevi V KitsiouEffie C Tsilibary
Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Klaas KoopJan A Bruijn

❮ Previous
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Citations

Aug 4, 2007·Kidney International. Supplement·J J LiS-W Kang
Jul 26, 2011·Advances in Chronic Kidney Disease·Puneet Garg, Ton Rabelink
May 6, 2008·Experimental Cell Research·Susana LarruceaRoberto Parrilla
Sep 12, 2015·Biochemical and Biophysical Research Communications·Feng GuoGuijun Qin
Feb 12, 2013·Biochemical and Biophysical Research Communications·Darío FernándezMatilde S Ayuso
Jun 25, 2016·Journal of Diabetes Research·Kaichiro SawadaMasafumi Fukagawa
Jul 21, 2016·PloS One·Elias N KatsoulierisEffie C Tsilibary
Mar 19, 2013·International Journal of Molecular Sciences·Kai LiZilong Li
Apr 26, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christina B SchroeterMarkus M Rinschen
Jul 11, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Rebecca J SteagallZhihua Han
Jul 7, 2009·Journal of the American Society of Nephrology : JASN·Julie S Nielsen, Kelly M McNagny
Feb 13, 2020·Cancers·Estíbaliz Tamayo-OrbegozoSusana Larrucea
Dec 17, 2017·Oncotarget·Estíbaliz Tamayo-OrbegozoSusana Larrucea
Mar 14, 2019·Clinical Kidney Journal·Maria Dolores Sanchez-NiñoAlberto Ortiz
Jul 3, 2021·Cancers·Ngoc Le TranGuiying Nie
Oct 20, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Lingfeng Zeng, Cheuk-Chun Szeto

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