Enhanced polyhydroxybutyrate (PHB) production via the coexpressed phaCAB and vgb genes controlled by arabinose P promoter in Escherichia coli

Letters in Applied Microbiology
Y-T HorngP-C Soo

Abstract

To develop an approach to enhance polyhydroxybutyrate (PHB) production via the coexpressed phaCAB and vgb genes controlled by arabinose P(BAD) promoter in Escherichia coli. The polyhydroxyalkanoates (PHAs) synthesis operon, (phaCAB), from Ralstonia eutropha was overexpressed under the regulation of the arabinose P(BAD) promoter in Escherichia coli, and the vgb gene encoding bacterial haemoglobin from Vitreoscilla stercoraria (VHb) was further cloned at downstream of phaCAB to form an artificial operon. The cell dry weight (CDW), PHB content and PHB concentration were enhanced around 1.23-, 1.57-, and 1.93-fold in the engineered cell harbouring phaCAB-vgb (SY-2) upon 1% arabinose induction compared with noninduction (0% arabinose). Furthermore, by using a recombinant strain harbouring P(BAD) promoter-vgb along with native promoter-phaCAB construction, the effect of vgb expression level on PHB biosynthesis was positive correlation. The results exploit the possibility to improve the PHB production by fusing the genes phaCAB-vgb from different species under the arabinose regulation system in E. coli. It also demonstrates that increase in VHb level enhances the PHB production. We were successful in providing a new coexpressed system...Continue Reading

References

Jan 1, 1997·Nature Biotechnology·S Y Lee
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·D A Siegele, J C Hu
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·L L Madison, G W Huisman
May 2, 2001·The Journal of Biological Chemistry· RamandeepD A Webster
Apr 6, 2002·Biotechnology Progress·Judith Farrés, Pauli T Kallio
Jun 25, 2002·The Journal of Biological Chemistry·Kyung-Won ParkDale A Webster
May 27, 2003·Bioresource Technology·C S K ReddyV C Kalia
Jul 2, 2003·Current Opinion in Microbiology·José M LuengoElías R Olivera
Jun 24, 2004·Biotechnology and Bioengineering·Meltem Urgun-DemirtasBenjamin C Stark
Jul 10, 2004·Cell·Sanie MnaimnehTimothy R Hughes
Nov 17, 2004·International Journal of Systematic and Evolutionary Microbiology·Peter Vandamme, Tom Coenye
Apr 10, 2007·Journal of Biotechnology·Chih-Ching ChienYu-Hong Wei
Mar 19, 2008·Applied Microbiology and Biotechnology·Zhen KangQingsheng Qi

❮ Previous
Next ❯

Citations

Jul 20, 2012·Applied Microbiology and Biotechnology·Seiki TakenoMasato Ikeda
Oct 29, 2013·Research in Microbiology·Po-Chi SooHsin-Chih Lai
Apr 7, 2018·Applied Microbiology and Biotechnology·Yilin RenGuo-Qiang Chen
Mar 23, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuanzhi HeJun Yuan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.