Enhanced polymer one-step staining for proliferating cell nuclear antigen in squamous cell carcinoma of the tongue

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
K SanoG Ma

Abstract

A rapid enhanced polymer one-step staining for proliferating cell nuclear antigen (EPOS-PCNA) in formalin fixed, paraffin embedded sections of tongue squamous cell carcinoma is described. EPOS-PCNA was compared with PCNA immunohistochemistry using the avidin-biotin complex (ABC) method, with or without autoclave pretreatment. Significant correlation of PCNA labeling index (percentage of labeled cells/total cells counted) was observed between EPOS-PCNA and the immunohistochemical protocol using autoclave pretreatment. We consider this method a reliable, timesaving technique that would be useful in quantitative histopathology and in performing double immunostaining.

References

Dec 1, 1992·International Journal of Oral and Maxillofacial Surgery·T TsujiF Shinozaki
Aug 1, 1990·The Journal of Pathology·A S Leong, J Milios
Jun 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S R ShiK L Kalra
Jan 1, 1989·American Journal of Clinical Pathology·S S Tabibzadeh, K D Shah
May 1, 1985·American Journal of Clinical Pathology·R V LloydB S Wilson
May 1, 1994·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·T Haerslev, G K Jacobsen
Oct 1, 1994·The Journal of Laryngology and Otology·A S JonesG Forster
Jan 1, 1994·Micron : the International Research and Review Journal for Microscopy·M E Boon, L P Kok

❮ Previous
Next ❯

Citations

Oct 10, 2013·Histopathology·Maria C ArgentieriGiorgio Cattoretti
May 8, 2004·Mechanisms of Ageing and Development·Katsuyasu KoudaSatoshi Sawada
Aug 19, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Kazuhisa HasuiFusayoshi Murata
Jul 26, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Amrita AhluwaliaAndrzej S Tarnawski
Dec 18, 2003·Journal of Experimental Therapeutics & Oncology·Seiichiro KaiSeigo Kitano

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.