Enhanced Prefrontal Neuronal Activity and Social Dominance Behavior in Postnatal Forebrain Excitatory Neuron-Specific Cyfip2 Knock-Out Mice

Frontiers in Molecular Neuroscience
Yinhua ZhangKihoon Han

Abstract

The cytoplasmic fragile X mental retardation 1 (FMR1)-interacting protein 2 (CYFIP2) gene is associated with epilepsy, intellectual disability (ID), and developmental delay, suggesting its critical role in proper neuronal development and function. CYFIP2 is involved in regulating cellular actin dynamics and also interacts with RNA-binding proteins. However, the adult brain function of CYFIP2 remains unclear because investigations thus far are limited to Cyfip2 heterozygous (Cyfip2+/- ) mice owing to the perinatal lethality of Cyfip2-null mice. Therefore, we generated Cyfip2 conditional knock-out (cKO) mice with reduced CYFIP2 expression in postnatal forebrain excitatory neurons (CaMKIIα-Cre). We found that in the medial prefrontal cortex (mPFC) of adult Cyfip2 cKO mice, CYFIP2 expression was decreased in both layer 2/3 (L2/3) and layer 5 (L5) neurons, unlike the L5-specific CYFIP2 reduction observed in adult Cyfip2+/- mice. Nevertheless, filamentous actin (F-actin) levels were increased only in L5 of Cyfip2 cKO mPFC possibly because of a compensatory increase in CYFIP1, the other member of CYFIP family, in L2/3 neurons. Abnormal dendritic spines on basal, but not on apical, dendrites were consistently observed in L5 neurons of ...Continue Reading

References

Jun 27, 2000·American Journal of Medical Genetics·S SprangerJ Bullerdiek
Nov 26, 2010·Nature·Zhucheng ChenMichael K Rosen
Aug 18, 2012·PloS One·Ozlem BozdagiJoseph D Buxbaum
Oct 9, 2012·Neuroscience Letters·Jinsoo SeoSe-Young Choi
Apr 16, 2014·Frontiers in Cellular Neuroscience·Sabiha Abekhoukh, Barbara Bardoni
Aug 28, 2014·Trends in Neurosciences·Fei WangHailan Hu
Oct 15, 2014·Molecular Psychiatry·A Oguro-AndoD H Geschwind
Dec 30, 2014·Frontiers in Systems Neuroscience·Danai RigaMichel C Van den Oever
Oct 11, 2015·The Journal of Biological Chemistry·Erin F Spence, Scott H Soderling
Feb 5, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kuangfu HsiaoDeanna L Benson
May 5, 2016·Nucleic Acids Research·Maxim V KuleshovAvi Ma'ayan
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hirobumi TadaTakuya Takahashi
Dec 27, 2016·Korean journal of pediatrics·Jin Hwan LeeGyung Min Lee
Sep 14, 2017·The Journal of Experimental Medicine·Myung-Su KangKyong-Tai Kim
Mar 14, 2018·Annals of Neurology·Mitsuko NakashimaNaomichi Matsumoto
Mar 25, 2018·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ryunhee KimEunjoon Kim
Apr 19, 2018·CNS Neuroscience & Therapeutics·Jing PengNan Pang
Oct 5, 2018·Proceedings of the National Academy of Sciences of the United States of America·Shawn TanH Shawn Je
Jan 22, 2019·Translational Psychiatry·Sven O BachmannStéphane J Baudouin
Feb 17, 2019·Nature Protocols·Zhengxiao FanHailan Hu
Mar 22, 2019·Neuron·Claudia Bagni, R Suzanne Zukin
Aug 3, 2019·Nature Communications·Nuria Domínguez-IturzaClaudia Bagni
Sep 12, 2019·Annals of Neurology·Yeunkum LeeKihoon Han

❮ Previous
Next ❯

Citations

Jul 31, 2021·Molecular Biology Reports·Ísis Venturi BiembengutPatrícia Shigunov

❮ Previous
Next ❯

Methods Mentioned

BETA
reverse transcription PCR
PCR
confocal microscopy
dissection
Fluorescence

Software Mentioned

GraphPad Prism
ImageJ
ZEN
Zeiss
EthoVision
pClamp
Enrichr

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.