Enhanced Pro-apoptotic Effects of Fe(II)-Modified IVIG on Human Neutrophils.

Frontiers in Immunology
Stefanie GraeterStephan von Gunten

Abstract

Mild modification of intravenous immunoglobulin (IVIG) has been reported to result in enhanced polyspecificity and leveraged therapeutic effects in animal models of inflammation. Here, we observed that IVIG modification by ferrous ions, heme or low pH exposure, shifted the repertoires of specificities in different directions. Ferrous ions exposed Fe(II)-IVIG, but not heme or low pH exposed IVIG, showed increased pro-apoptotic effects on neutrophil granulocytes that relied on a FAS-dependent mechanism. These effects were also observed in human neutrophils primed by inflammatory mediators or rheumatoid arthritis joint fluid in vitro, or patient neutrophils ex vivo from acute Crohn's disease. These observations indicate that IVIG-mediated effects on cells can be enhanced by IVIG modification, yet specific modification conditions may be required to target specific molecular pathways and eventually to enhance the therapeutic potential.

References

Jun 12, 1975·The New England Journal of Medicine·T B StromG J Busch
Aug 7, 1986·The New England Journal of Medicine·J W NewburgerS P Sanders
Nov 13, 2001·Clinical and Experimental Immunology·H TsujimotoI Sekine
May 25, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Hiroshi TsujimotoIsao Sekine
Mar 1, 2003·Science·Leo C JamesDan S Tawfik
Dec 6, 2003·The Journal of Allergy and Clinical Immunology·Frank AltznauerHans-Uwe Simon
Oct 26, 2005·The Journal of Biological Chemistry·Jordan D DimitrovTchavdar L Vassilev
Jul 20, 2007·The Journal of Biological Chemistry·Jordan D DimitrovTchavdar L Vassilev
May 16, 2009·The Journal of Allergy and Clinical Immunology·Stephan von GuntenBruce S Bochner
Oct 9, 2009·Autoimmunity·Iglika K Djoumerska-AlexievaTchavdar L Vassilev
Apr 21, 2010·Journal of Clinical Immunology·Stephan von Gunten, Hans-Uwe Simon
Dec 3, 2010·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Sladjana PavlovicMiodrag L Lukic
Nov 23, 2012·The New England Journal of Medicine·Erwin W Gelfand
May 15, 2013·Trends in Immunology·Barbara GeeringHans-Uwe Simon
Jan 28, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jing-Rong HuangAlice L Yu
Apr 9, 2014·Proceedings of the National Academy of Sciences of the United States of America·Oliver M T PearceAjit Varki
Apr 26, 2014·Nature Reviews. Immunology·Stephan von GuntenHans-Uwe Simon
May 13, 2014·Nature Chemical Biology·Sean R StowellRichard D Cummings
Oct 24, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marc WehrliStephan von Gunten
Sep 18, 2016·Archivum Immunologiae Et Therapiae Experimentalis·Peter J SpäthStephan von Gunten
Nov 1, 2016·Inflammation·Iglika Djoumerska-AlexievaJordan D Dimitrov
Dec 9, 2016·Human Vaccines & Immunotherapeutics·Shina PashovaAnastas Pashov
Dec 22, 2016·Journal of Clinical Medicine·Odile WéraCécile Oury
May 4, 2017·Scientific Reports·Christoph SchneiderStephan von Gunten
Jan 27, 2018·Cell Death & Disease·Xiaoliang WangHans-Uwe Simon
Feb 6, 2019·Nature Reviews. Immunology·Alexia KanyavuzJordan D Dimitrov
Feb 10, 2019·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Stefanie GraeterStephan von Gunten
Oct 28, 2019·Frontiers in Immunology·Chih-Chao ChiangTsong-Long Hwang

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Methods Mentioned

BETA
chemical modification
blood drawn
FCS
flow cytometry
dot blot
ELISA
array technology

Software Mentioned

GraphPad
GraphPad Prism
R Foundation for Statistical Computing
R

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