Enhanced pro-inflammatory cytokine production in Galphai2-deficient mice on colitis prone and colitis resistant 129Sv genetic backgrounds

Cellular Immunology
Malin BjurstenElisabeth Hultgren Hörnquist

Abstract

Mice deficient in G-protein subunit alphai2 develop colitis closely resembling human ulcerative colitis when raised on 129SvEv background. When backcrossing the Galphai2-deficiency into a 129SvJBom genetic background, surprisingly, mice did not develop colitis. In vitro stimulation of splenocytes with formalin-killed Staphylococcus aureus resulted in significantly increased production of interleukin-1beta, tumor necrosis factor, and interleukin-12p40 in Galphai2(-/-) as compared to control mice. The enhanced production of pro-inflammatory cytokines was seen in colitis prone as well as in colitis resistant genetic background. A similar outcome was seen upon stimulation with toxic shock syndrome toxin-1, a T cell superantigen, except that Galphai2(-/-) colitis resistant 129SvJBom splenocytes did not show increased production of IL-12p40 as compared to their controls.

References

Nov 1, 1990·Arthritis and Rheumatism·T BremellA Tarkowski
Sep 1, 1996·The Journal of Experimental Medicine·M KopfW Solbach
Jul 24, 2003·Nature Reviews. Immunology·Gerd Bouma, Warren Strober
Dec 10, 2003·The Journal of Experimental Medicine·Craig A MurphyDaniel J Cua

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Citations

Apr 9, 2013·Archivum Immunologiae Et Therapiae Experimentalis·Yantang WangGuixiu Shi
Oct 22, 2005·Inflammatory Bowel Diseases·Malin BjurstenElisabeth Hultgren Hörnquist
Feb 25, 2009·PloS One·Gwang LeeSangdun Choi
Feb 20, 2008·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Bo WeiJonathan Braun
Mar 25, 2005·American Journal of Physiology. Cell Physiology·Hongkuan FanJames A Cook
Apr 27, 2011·International Journal of Cancer. Journal International Du Cancer·Aya M WestbrookRobert H Schiestl
Feb 21, 2006·World Journal of Gastroenterology : WJG·O H HultgrenE Hultgren Hörnquist

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