Enhanced production and gene expression of interleukin-5 in patients with bronchial asthma: possible management of atopic diseases by down-regulation of interleukin-5 gene transcription

International Archives of Allergy and Immunology
H OkudairaK Ito

Abstract

Interleukin (IL)-5 was produced in vitro by peripheral blood mononuclear cells (PBMCs) of mite-sensitive atopic patients upon challenge with specific allergen, while PBMCs of healthy controls produced essentially no IL-5. Stimuli delivered by the combination of phorbol ester (PMA) and CA2+ ionophore (ionomycin) induced marked IL-5 production by PBMCs obtained from atopic as well as nonatopic asthmatics. CD2- or CD4-bearing-cell depletion almost completely removed IL-5 production and gene transcription while CD8 depletion did not. These findings indicated that CD4+ T cells are the principal source of IL-5 in asthmatic PBMCs. The capacity of PBMCs of atopic asthmatics, nonatopic asthmatics and healthy controls to produce IL-2, IL-3, IL-4, interferon-gamma and granulocyte/macrophage-colony-stimulating factor was comparable among the three groups. FK506 suppressed IL-5 production and gene expression in vitro in a dose-dependent manner.

Citations

Dec 1, 1995·Current Opinion in Immunology
Oct 6, 1997·Journal of the American Academy of Dermatology·R M TrüebG Burg
Jan 1, 1997·Research in Immunology·J A Lasky, A R Brody
Mar 1, 1997·Allergy·A Ruffilli, S Bonini
Jan 1, 1997·Memórias do Instituto Oswaldo Cruz·A T Kotsimbos, Q Hamid
Mar 20, 1998·International Reviews of Immunology·S KarlenC J Sanderson
May 21, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T NomaN Matsuura
Apr 1, 1996·Immunology and Cell Biology·S KarlenC J Sanderson
Mar 8, 1999·Molecular Cell Biology Research Communications : MCBRC·T KohyamaK Yamamoto

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.