Enhanced production and immunological characterization of recombinant West Nile virus envelope domain III protein

New Biotechnology
Nagesh K TripathiJyoti S Kumar

Abstract

West Nile virus (WNV) is an emerging mosquito-borne virus which is responsible for severe and fatal encephalitis in humans and for which there is no licensed vaccine or therapeutic available to prevent infection. The envelope domain III protein (EDIII) of WNV was over-expressed in Escherichia coli and purified using a two-step chromatography process which included immobilized metal affinity chromatography and ion exchange chromatography. E. coli cells were grown in a bioreactor to high density using batch and fed-batch cultivation. Wet biomass obtained after batch and fed-batch cultivation processes was 11.2 g and 84 g/L of culture respectively. Protein yield after affinity purification was 5.76 mg and 5.81 mg/g wet cell weight after batch and fed-batch processes respectively. The purified WNV EDIII elicited specific antibodies in rabbits, confirming its immunogenicity. Moreover, the antibodies were able to neutralize WNV in vitro. These results established that the refolded and purified WNV EDIII could be a potential vaccine candidate.

References

Jun 9, 2004·Journal of Clinical Microbiology·David W C BeasleyAlan D T Barrett
Oct 1, 2005·Journal of Industrial Microbiology & Biotechnology·Daniel MandersonYusuf Chisti
Aug 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bärbel KaufmannMichael G Rossmann
Nov 15, 2006·Applied Microbiology and Biotechnology·Jolanta SereikaiteVladas-Algirdas Bumelis
Feb 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jang-Hann J ChuMah-Lee Ng
Oct 8, 2008·Immunological Reviews·Michael S DiamondDaved H Fremont
Apr 30, 2009·PloS One·Qi Ying KooJ Thomas August
Jul 2, 2010·Journal of Industrial Microbiology & Biotechnology·Surianarayanan BhuvaneshSubramanian Ramalingam
Jan 19, 2012·Journal of Industrial Microbiology & Biotechnology·Chung-Jr HuangXiaoming Yang
Jan 30, 2013·Diagnostic Microbiology and Infectious Disease·Divyasha SaxenaJyoti S Kumar
Apr 3, 2014·Expert Review of Vaccines·Ian J Amanna, Mark K Slifka
Apr 19, 2015·Microbial Cell Factories·Anupam SinghAmulya Kumar Panda
Feb 11, 2016·Microbial Cell Factories·Laura Sanchez-GarciaAntonio Villaverde
Apr 14, 2017·AIMS Microbiology·Nagesh K Tripathi, Ambuj Shrivastava

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Citations

Aug 2, 2019·The Annals of Otology, Rhinology, and Laryngology·Daniela ParrinoGino Marioni
Sep 8, 2018·Frontiers in Immunology·Nagesh K Tripathi, Ambuj Shrivastava
May 26, 2021·Human Vaccines & Immunotherapeutics·Mark VerdeciaFouad Atouf

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