Enhanced production of 2,3-butanediol by engineered Bacillus subtilis

Applied Microbiology and Biotechnology
Ranjita BiswasAkihiko Kondo

Abstract

Production of 2,3-butanediol by Bacillus subtilis takes place in late-log or stationary phase, depending on the expression of bdhA gene encoding acetoin reductase, which converts acetoin to 2,3-butanediol. The present work focuses on the development of a strain of B. subtilis for enhanced production of 2,3-butanediol in early log phase of growth cycle. For this, the bdhA gene was expressed under the control of P( alsSD ) promoter of AlsSD operon for acetoin fermentation which served the substrate for 2,3-butanediol production. Addition of acetic acid in the medium induced the production of 2,3-butanediol by 2-fold. Two-step aerobic-anaerobic fermentation further enhanced 2,3-butanediol production by 4-fold in comparison to the control parental strain. Thus, addition of acetic acid and low dissolved oxygen in the medium are involved in activation of bdhA gene expression from P( alsSD ) promoter in early log phase. Under the conditions tested in this work, the maximum production of 2,3-butanediol, 2.1 g/l from 10 g/l glucose, was obtained at 24 h. Furthermore, under the optimized microaerophilic condition, the production of 2,3-butanediol improved up to 6.1 g/l and overall productivity increased by 6.7-fold to 0.4 g/l h in the en...Continue Reading

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Citations

Jun 1, 2013·Applied Microbiology and Biotechnology·Ileana-Maria JurchescuSiegmund Lang
Jul 10, 2013·Journal of Industrial Microbiology & Biotechnology·Xian ZhangZhiming Rao
Jul 28, 2013·Microbial Cell Factories·Johannes KabischThomas Schweder
Jun 1, 2014·Journal of Industrial Microbiology & Biotechnology·Songsong GaoHongjiang Yang
Jan 31, 2016·Applied Biochemistry and Biotechnology·Apoorva Nandkumar DeshmukhRishi Jain
Feb 3, 2016·Applied Microbiology and Biotechnology·Kosuke TanimuraAkihiko Kondo
Sep 20, 2012·New Biotechnology·Sung-Hyuk HanYong-Cheol Park
Oct 12, 2015·Applied Microbiology and Biotechnology·Rafael R de Oliveira, Wayne L Nicholson
Jun 30, 2015·Bioscience, Biotechnology, and Biochemistry·Yoshihiro ToyaHiroshi Shimizu
Oct 9, 2015·Journal of Industrial Microbiology & Biotechnology·Aneta M BiałkowskaMarianna Turkiewicz
Nov 23, 2015·Applied Microbiology and Biotechnology·Aneta M BiałkowskaMarianna Turkiewicz
Oct 15, 2013·Journal of Biotechnology·Tao ChenYa-jie Tang
Jan 15, 2015·Applied Biochemistry and Biotechnology·Jian-Ying DaiZhi-Long Xiu
Jun 4, 2016·Biotechnology for Biofuels·Yimin QiuShouwen Chen
Oct 26, 2016·World Journal of Microbiology & Biotechnology·Aneta M Białkowska
Oct 16, 2015·Preparative Biochemistry & Biotechnology·Barbara SikoraMarianna Turkiewicz
Nov 11, 2016·Engineering in Life Sciences·Julian LangeBastian Blombach
Dec 13, 2016·Bioresources and Bioprocessing·Xin LvYaling Shen
Dec 6, 2020·Journal of Bioscience and Bioengineering·Asuka MizobataHiroyasu Ogino

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