Enhanced production of a diastereomer type of mannosylerythritol lipid-B by the basidiomycetous yeast Pseudozyma tsukubaensis expressing lipase genes from Pseudozyma antarctica

Applied Microbiology and Biotechnology
Azusa SaikaTomotake Morita

Abstract

Basidiomycetous yeasts in the genus Pseudozyma are known to produce extracellular glycolipids called mannosylerythritol lipids (MELs). Pseudozyma tsukubaensis produces a large amount of MEL-B using olive oil as the sole carbon source (> 70 g/L production). The MEL-B produced by P. tsukubaensis is a diastereomer type of MEL-B, which consists of 4-O-β-D-mannopyranosyl-(2R,3S)-erythritol as a sugar moiety, in contrast to the conventional type of MELs produced by P. antarctica, which contain 4-O-β-D mannopyranosyl-(2S,3R)-erythritol. In this study, we attempted to increase the production of the diastereomer type of MEL-B in P. tsukubaensis 1E5 by introducing the genes encoding two lipases, PaLIPAp (PaLIPA) and PaLIPBp (PaLIPB) from P. antarctica T-34. Strain 1E5 expressing PaLIPA exhibited higher lipase activity than the strain possessing an empty vector, which was used as a negative control. Strains of 1E5 expressing PaLIPA or PaLIPB showed 1.9- and 1.6-fold higher MEL-B production than the negative control strain, respectively, and oil consumption was also accelerated by the introduction of these lipase genes. MEL-B production was estimated using time course analysis in the recombinant strains. Strain 1E5 expressing PaLIPA produc...Continue Reading

References

Mar 9, 1999·Antonie van Leeuwenhoek·S Lang, J C Philp
Sep 28, 1999·Applied Microbiology and Biotechnology·E Rosenberg, E Z Ron
Dec 29, 2000·Applied Microbiology and Biotechnology·R M Maier, G Soberón-Chávez
Oct 11, 2003·Biotechnology Advances·R SharmaU C Banerjee
Jul 13, 2004·Applied Microbiology and Biotechnology·U RauS Lang
Feb 25, 2005·Applied Microbiology and Biotechnology·U RauS Lang
Jan 25, 2008·Journal of Bioscience and Bioengineering·Tomotake MoritaDai Kitamoto
May 1, 2010·Journal of Oleo Science·Tomotake MoritaDai Kitamoto
Jul 14, 2010·Journal of Oleo Science·Tomotake MoritaDai Kitamoto
Jul 14, 2012·Journal of Oleo Science·Shuhei YamamotoMasaru Kitagawa

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Datasets Mentioned

BETA
JCM16987

Methods Mentioned

BETA
acetylation
PCR
PaLIPA
Assay
electrophoresis
light scattering

Software Mentioned

BLASTP
PaLIPB
PaLIPA

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