Enhanced production of d-lactic acid by Sporolactobacillus sp.Y2-8 mutant generated by atmospheric and room temperature plasma

Biotechnology and Applied Biochemistry
Jiaduo SunBingfang He

Abstract

To improve the production of d-lactic acid, atmospheric and room temperature plasma (ARTP) was used to generate mutations in Sporolactobacillus sp. Y2-8. An efficient mutant YBS1-5 was rapidly isolated by implanting ARTP twice with a 100 W radio-frequency power input, 10 standard liters per minute of the helium flow, and a 2 mm treatment distance. Significant improvement of d-lactic acid productivity (1.39 g L(-1) H(-1) ) by YBS1-5 was achieved, and it was 41.84% higher than the productivity (0.98 g L(-1) H(-1) ) of Y2-8. Moreover, the dry cell weight of YBS1-5 was 16.7% higher than that of Y2-8. Metabolic activities of concerned substrates related with key enzymes of d-lactic acid fermentation were analyzed by Biolog approach. Results showed that the activities of the key enzymes glucokinase and d-lactate dehydrogenase in mutant YBS1-5 were increased by approximately 45% and 66%, respectively, in comparison with those of the strain Y2-8. Fed-batch fermentation further improved the productivity; 127 g L(-1) d-lactic acid in 74 H by YBS1-5 with higher productivity (1.72 g L(-1) H(-1) ) was achieved. The subculture experiments indicated that YBS1-5 was genetically stable after eight generations.

References

Jan 12, 2008·Applied Microbiology and Biotechnology·Shohei OkinoHideaki Yukawa
Dec 25, 2009·Biotechnology Letters·D S JoshiD V Gokhale
Mar 2, 2011·Applied Biochemistry and Biotechnology·Luciana Fontes CoelhoJonas Contiero
Jun 12, 2012·Metabolic Engineering·Li ZhouZheng-Xiang Wang

Citations

Oct 2, 2015·Applied Biochemistry and Biotechnology·Qingxin LiJin Chuan Wu
Sep 23, 2016·Applied Microbiology and Biotechnology·Silvia KlotzUlf Prüße
Jan 11, 2020·Applied Microbiology and Biotechnology·Qinghui YuGuoquan Hu
May 29, 2018·Journal of Industrial Microbiology & Biotechnology·Shaoxiong LiangJianping Wen

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