Abstract
Saccharomyces cerevisiae is a popular organism for metabolic engineering; however, studies aiming at over-production of bio-replacement precursors for the chemical industry often fail to overcome proof-of-concept stage. When intending to show real industrial attractiveness, the challenge is twofold: formation of the target compound must be increased, while minimizing the formation of side and by-products to maximize titer, rate and yield. To tackle these, the metabolism of the organism, as well as the parameters of the process, need to be optimized. Addressing both we show that S. cerevisiae is well-suited for over-production of aromatic compounds, which are valuable in chemical industry and are particularly useful in space technology. Specifically, a strain engineered to accumulate chorismate was optimized for formation of para-hydroxybenzoic acid. Then a fed-batch bioreactor process was developed, which delivered a final titer of 2.9 g/L, a maximum rate of 18.625 mgpHBA/(gCDW × h) and carbon-yields of up to 3.1 mgpHBA/gglucose.
References
Aug 1, 1983·Perceptual and Motor Skills·P J Locher, D L Bigelow
Apr 1, 1994·Microbiology·M SiebertL Heide
Dec 18, 2001·Biotechnology and Bioengineering·J L Barker, J W Frost
Feb 5, 2002·Biochimica Et Biophysica Acta·M J HoldenV L Vilker
Sep 29, 2007·Journal of Biotechnology·Suzanne VerhoefJan Wery
Mar 29, 2008·Metabolic Engineering·M A H LuttikJ M Daran
Jul 2, 2009·FEMS Yeast Research·Derek A AbbottAntonius J A van Maris
Jul 14, 2010·Yeast·Siavash PartowJérôme Maury
May 15, 2012·Journal of Biotechnology·Jens O KrömerCristian Varela
Aug 17, 2012·Nature·Pamela P Peralta-YahyaJay D Keasling
Sep 4, 2013·New Biotechnology·Suzanne VerhoefJohannes H de Winde
Jul 2, 2014·Yeast·Gal WinterJens O Krömer
Mar 21, 2015·Metabolic Engineering·T C WilliamsJ O Krömer
Aug 21, 2015·Metabolic Engineering·Angelica RodriguezJens Nielsen
May 28, 2016·Microbial Cell Factories·Nils J H AvereschJens O Krömer
Aug 6, 2016·Biotechnology Journal·Bin LaiJens O Krömer
Aug 17, 2016·Biochemical Society Transactions·Lynn J Rothschild
Dec 15, 2016·Frontiers in Bioengineering and Biotechnology·Shiqin YuJens O Krömer
Citations
Feb 2, 2019·Organic & Biomolecular Chemistry·Mads J GrüningerJennifer N Andexer
Jun 11, 2020·Biomolecules·Egle ValancieneNaglis Malys
Sep 30, 2020·Microbial Biotechnology·Huayi LiuYunzi Luo
Apr 11, 2018·Frontiers in Bioengineering and Biotechnology·Nils J H Averesch, Jens O Krömer
Aug 21, 2018·Frontiers in Microbiology·Nicolai Kallscheuer
Jan 24, 2020·Frontiers in Bioengineering and Biotechnology·Sara Castaño-CerezoGilles Truan
Jun 2, 2020·Frontiers in Bioengineering and Biotechnology·Jens O KrömerNorbert Kohlheb
Mar 11, 2020·Frontiers in Bioengineering and Biotechnology·Adelaide Braga, Nuno Faria
Aug 14, 2019·Metabolic Engineering·Mingfeng CaoZengyi Shao
Apr 15, 2020·Biotechnology Advances·Meijie LiHaibo Zhang