Enhanced production of spinosad in Saccharopolyspora spinosa by genome shuffling

Applied Biochemistry and Biotechnology
Z H JinP L Cen

Abstract

Spinosad (spinosyns A and D) is a mixture of secondary metabolites produced by Saccharopolyspora spinosa. It is used in agriculture as a potent insect control agent with exceptional safety to non-target organisms. In this study, we applied genome shuffling of S. spinosa to achieve a rapid improvement of spinosad production. Ten strains with subtle improvements in spinosad production were obtained from the populations generated by the mutation with nitrosoguanidine and ultraviolet irradiation, and then they were subjected for recursive protoplast fusion. After four rounds of genome shuffling, a high yielding strain, designated as S. spinosa 4-7, was successfully isolated. Its production reached 547 mg/L, which was increased by 200.55% and 436.27% in comparison with that of the highest parent strain and the original strain, respectively. The subculture experiments indicated that the high producer of S. spinosa 4-7 was stable. Spinosad fermentation experiments by S. spinosa 4-7 were carried out in a 5-L fermentor, and its production of spinosad reached 428 mg/L after 168 h of fermentation.

References

Feb 1, 1991·Journal of Paediatrics and Child Health·P J CampbellJ M Gupta
Apr 18, 1985·Nature·D A HopwoodS Omura
Oct 13, 2000·Applied Microbiology and Biotechnology·S ParekhR J Strobel
Jan 5, 2002·Journal of Industrial Microbiology & Biotechnology·K MadduriR H Baltz
Feb 8, 2002·Nature·Ying-Xin ZhangStephen B del Cardayré
Jun 29, 2002·Nature Biotechnology·Ranjan PatnaikStephen del Cardayré
Nov 10, 2004·Pest Management Science·Michel P BlancLuca Rossi
Oct 18, 2006·Applied Microbiology and Biotechnology·Hiroyuki HidaYasuhiro Yamada

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Citations

Jan 25, 2012·Journal of Industrial Microbiology & Biotechnology·Ge JingpingPing Wenxiang
May 24, 2013·Applied Biochemistry and Biotechnology·Chao WangXinle Liang
Oct 1, 2013·BioMed Research International·Amit Kumar ChaudharyJae Kyung Sohng
Mar 7, 2014·Applied Microbiology and Biotechnology·Damien Biot-Pelletier, Vincent J J Martin
May 26, 2009·Biotechnology Advances·Jixian GongXueming Zhao
Jan 8, 2014·Microbial Biotechnology·Jun ShiHuiping Deng
Jul 13, 2016·Applied and Environmental Microbiology·Jun HuangYu-Guo Zheng
Jun 24, 2019·Journal of Applied Microbiology·A M SayedU R Abdelmohsen
Apr 2, 2018·Biotechnology Letters·Fangxue XuChunchao Han
Oct 24, 2019·World Journal of Microbiology & Biotechnology·Ping YuTingting Yan
May 23, 2012·Journal of Agricultural and Food Chemistry·Jian-Mei LuoMin Wang
Oct 2, 2021·Critical Reviews in Food Science and Nutrition·Ravichandra HospetMisbah Mubeen Tallur

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