Enhanced production of steviol glycosides in mycorrhizal plants: a concerted effect of arbuscular mycorrhizal symbiosis on transcription of biosynthetic genes

Plant Physiology and Biochemistry : PPB
Shantanu MandalRupam Kapoor

Abstract

Stevia rebaudiana (Bertoni) produces steviol glycosides (SGs)--stevioside (stev) and rebaudioside-A (reb-A) that are valued as low calorie sweeteners. Inoculation with arbuscular mycorrhizal fungi (AMF) augments SGs production, though the effect of this interaction on SGs biosynthesis has not been studied at molecular level. In this study transcription profiles of eleven key genes grouped under three stages of the SGs biosynthesis pathway were compared. The transcript analysis showed upregulation of genes encoding 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway enzymes viz.,1-deoxy-D-xylulose 5-phospate synthase (DXS), 1-deoxy-D-xylulose 5-phospate reductoisomerase (DXR) and 2-C-methyl-D-erytrithol 2,4-cyclodiphosphate synthase (MDS) in mycorrhizal (M) plants. Zn and Mn are imperative for the expression of MDS and their enhanced uptake in M plants could be responsible for the increased transcription of MDS. Furthermore, in the second stage of SGs biosynthesis pathway, mycorrhization enhanced the transcription of copalyl diphosphate synthase (CPPS) and kaurenoic acid hydroxylase (KAH). Their expression is decisive for SGs biosynthesis as CPPS regulates flow of metabolites towards synthesis of kaurenoid precursors and KAH direc...Continue Reading

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Citations

Oct 27, 2015·Plant Biology·M T WellingK Benkendorff
Dec 6, 2017·The Open Microbiology Journal·Emanuela Lima Dos SantosFábio Sérgio Barbosa da Silva
Nov 22, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Silvia TavariniLuciana G Angelini
Apr 30, 2021·Applied Microbiology and Biotechnology·Marta Libik-KoniecznyRobert Konieczny
Jul 2, 2021·Applied Microbiology and Biotechnology·Xuan ZhouLong Liu

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