Enhanced production of succinic acid from methanol-organosolv pretreated Strophanthus preussii by recombinant Escherichia coli

Bioprocess and Biosystems Engineering
Ayobami Matthew OlajuyinJian-min Xing

Abstract

A biorefinery process for high yield production of succinic acid from biomass sugars was investigated using recombinant Escherichia coli. The major problem been addressed is utilization of waste biomass for the production of succinic acid using metabolic engineering strategy. Here, methanol extract of Strophanthus preussii was used for fermentation. The process parameters were optimized. Glucose (9 g/L), galactose (4 g/L), xylose (6 g/L) and arabinose (0.5 g/L) were the major sugars present in the methanol extract of S. preussii. E. coli K3OS with overexpression of soluble nucleotide pyridine transhydrogenase sthA and mutation of lactate dehydrogenase A (ldhA), phosphotransacetylase acetate kinase A (pta-ackA), pyruvate formate lyase B (pflB), pyruvate oxidase B (poxB), produced a final succinic acid concentration of 14.40 g/L and yield of 1.10 mol/mol total sugars after 72 h dual-phase fermentation in M9 medium. Here, we show that the maximum theoretical yield using methanol extracts of S. preussii was 64%. Hence, methanol extract of S. preussii could be used for the production of biochemicals such as succinate, malate and pyruvate.

References

Sep 1, 1994·Molecular Microbiology·M H Saier, J Reizer
Nov 1, 1995·Archives of Microbiology·G A Sprenger
Apr 5, 1996·Journal of Molecular Biology·H Müller-Hartmann, B Müller-Hill
Feb 19, 2002·Environmental Toxicology·Min-Yu ChenMasanori Fujita
Mar 7, 2006·Applied and Environmental Microbiology·Sang Jun LeeSang Yup Lee
Jul 5, 2007·Applied Microbiology and Biotechnology·James B McKinlayJ Gregory Zeikus
Jan 15, 2008·Trends in Biotechnology·Michael SauerPaola Branduardi
Sep 11, 2008·Biotechnology and Bioengineering·Kaemwich JantamaL O Ingram
Feb 14, 2009·Applied Microbiology and Biotechnology·Xuebing ZhaoDehua Liu
Nov 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xueli ZhangLonnie O Ingram
Nov 3, 2010·Metabolic Engineering·Amarjeet SinghRyan T Gill
Nov 5, 2011·Critical Reviews in Toxicology·Gwendolyn L BallVirunya S Bhat
Aug 28, 2012·Journal of Bacteriology·Sonja Steinsiek, Katja Bettenbrock
Nov 20, 2015·Biotechnology for Biofuels·Michael F A BradfieldWillie Nicol

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