Enhanced pseudotargeted analysis using a segment data dependent acquisition strategy by LC-MS/MS for a metabolomics study of liquiritin in the treatment of depression

Journal of Separation Science
Jina YangTing Zhou

Abstract

An enhanced pseudotargeted method using a segment data dependent acquisition mode based on ultra-high performance liquid chromatography-mass spectrometry was developed. This segment data dependent acquisition-based pseudotargeted method could improve the detection of co-eluted ions and extend the coverage of analytes. A set of 502 multiple reaction monitoring channels were obtained by this segment strategy, which was twice the number created by the traditional data dependent acquisition mode. Compared with the untargeted method, the pseudotargeted profiling demonstrated higher sensitivity and higher precision. More than 90% of the metabolites detected by the enhanced pseudotargeted method had RSDs less than 15%. The segment data dependent acquisition-based pseudotargeted method was successfully applied to the metabolomics study of the depressed rats with the treatment of liquiritin. Forty-seven differential metabolites were screened and five metabolic pathways were found to be relate to depression including retinol metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, terpenoid backbone biosynthesis and lysine degradation. The segment data dependent acquisition-based pseudotargeted method wi...Continue Reading

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