Enhanced rates of fluid pinocytosis during exponential growth and monolayer regeneration by cultured arterial endothelial cells

Journal of Cellular Physiology
P F DaviesS M Schwartz

Abstract

Rates of fluid pinocytosis by bovine aortic endothelial cells were measured during various manipulations of growth status in vitro. Sparsely seeded cultures grew exponentially until a confluent monolayer was formed, at which time growth slowed. This change in growth rate coincided with a decline in the rate of pinocytosis to about one-third that in the growing cultures. During the subsequent attainment of maximal cell density in the confluent monolayer, the pinocytic rate remained constant. There was close correlation between 3H-thymidine labelling indices, as measured by autoradiography, and the rates of pinocytosis. Mechanical "wounding" of the confluent monolayer resulted in cell migration and proliferation. Twenty-four hours after "wounding," rates of pinocytosis per mg. cell protein were significantly enhanced. When regeneration of the monolayer was blocked by cytochalasin B, pinocytosis remained at the same rate as in the uninjured, confluent monolayer. These experiments support, and extend to endothelium, earlier observations that in growing cells pinocytosis proceeds at a higher rate than in non-growing, quiescent cells. Furthermore, they raise the possibility that the transendothelial transport of macromolecules such a...Continue Reading

References

Jan 1, 1977·Annual Review of Biochemistry·J L Goldstein, M S Brown
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·I VlodavskyD Gospodarowicz
Jun 23, 1978·Biochimica Et Biophysica Acta·J P RecklessD Steinberg
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C R MinickW Insull
May 1, 1979·The Journal of Cell Biology·S C Selden, S M Schwartz
Aug 1, 1978·Journal of Cellular Physiology·R T WallG E Striker
Aug 1, 1977·Circulation Research·S M Schwartz, E P Benditt
Mar 1, 1976·Experimental Cell Research·C C HaudenschildM Klagsbrun
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S M Schwartz, E P Benditt
Jan 20, 1970·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·L Florey, B L Sheppard
Oct 1, 1972·The Journal of Experimental Medicine·M B Stemerman, R Ross
May 1, 1973·The Journal of Cell Biology·N SimionescuG E Palade

❮ Previous
Next ❯

Citations

Oct 1, 1984·Atherosclerosis·P Görög, J D Pearson
Jun 8, 2002·Advanced Drug Delivery Reviews·Francesco M VeroneseMauro Sergi
Aug 1, 1992·Neuropathology and Applied Neurobiology·J T BeswetherickG Allt
Apr 1, 1993·Acta Pathologica Japonica·M OhashiJ Handa
Jan 1, 1992·Diabetic Medicine : a Journal of the British Diabetic Association·R G Petty, J D Pearson
Nov 1, 1988·The American Review of Respiratory Disease·M L Render, S Rounds
Jan 1, 1986·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·A TanimuraM Kitazono
Apr 1, 1982·Experimental and Molecular Pathology·D S LeakeD E Bowyer
May 1, 1983·Neuropathology and Applied Neurobiology·P H AbrahamsG Allt
Apr 1, 1982·European Journal of Clinical Investigation·U SchöffelC Mittermayer
Sep 14, 2015·Journal of Pharmacokinetics and Pharmacodynamics·Patrick M GlassmanJoseph P Balthasar
Aug 1, 1981·Experimental Cell Research·P F Davies
Jun 1, 1989·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A Baranczyk-KuzmaK L Audus
Mar 1, 1981·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·S M SchwartzS C Selden
Nov 1, 1983·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·V W van HinsberghM Scheffer
Sep 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·L N Walker, D E Bowyer
Jul 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·R KenagyJ J Albers
Jan 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·R R Schleef, C R Birdwell
May 1, 1982·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·M A ReidyS M Schwartz
Sep 10, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·J X RongA Sevanian
Dec 16, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·J X RongA Sevanian
Aug 1, 1981·Journal of Cellular Physiology·S C SeldenS M Schwartz
Sep 1, 1981·Journal of Cellular Physiology·P J Del Vecchio, J R Smith
Jan 1, 1980·Journal of Supramolecular Structure·P F Davies
Jul 10, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S E ChowJ K Chen
Oct 1, 1986·Brain Research·R A RobinsonM N Hart

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