Enhanced recovery of low concentration protein and peptide solutions on ultra-low binding microplates

Future Science OA
Christopher M WeikartBrian R Maurer

Abstract

SiO2 Medical Products (SIO) developed PureWARE™ Ultra-Low Binding (ULB) plasma-treated microplates with the combined benefits of enhanced protein recovery and reduced extractables. This study demonstrates enhanced protein recoveries, but at ten-times lower protein concentration, or 0.1 nM, compared with a prior study. In addition, no significant effect on enhanced protein recovery of plasma-treated microplates was observed in a long-term stability study carried out for 26 months under ambient storage conditions. Furthermore, recovery of three different peptide solutions, in the concentration range of 1.5-12 nM, was also shown to be enhanced on plasma-treated microplates relative to standard polypropylene microplates.

References

Feb 8, 2011·Advances in Colloid and Interface Science·Michael RabeStefan Seeger
Feb 15, 2011·Analytical Biochemistry·Miriam Goebel-StengelJoseph R Reeve
May 2, 2015·PloS One·Kasper KristensenThomas L Andresen
Apr 19, 2017·PDA Journal of Pharmaceutical Science and Technology·Christopher M WeikartJeff R Shallenberger

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Methods Mentioned

BETA
fluorescence spectroscopy
surface plasmon resonance

Software Mentioned

PureWARE

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