Enhanced removal of detergent and recovery of enzymatic activity following sodium dodecyl sulfate-polyacrylamide gel electrophoresis: use of casein in gel wash buffer

Analytical Biochemistry
B R McGrew, D M Green

Abstract

The inclusion of 1% casein or bovine serum albumin in buffer used to reactivate enzymes subjected to sodium dodecyl sulfate (SDS)-polyacrylamide electrophoresis resulted in accelerated removal of SDS and restoration of nuclease and beta-galactosidase enzyme activities. Nuclease and beta-galactosidase activities which are absent from gels after longer wash procedures are detectable with this technique. Enzyme activity in gels prepared with SDS which contained inhibitory contaminants was partially restored by the casein wash procedure. The threshold of detection of two-dimensionally separated deoxyribonuclease I using the casein wash procedure was 1 picogram.

References

May 15, 1977·Analytical Biochemistry·A L Rosenthal, S A Lacks
Sep 1, 1983·Analytical Biochemistry·S A Coughlin, D M Green

❮ Previous
Next ❯

Citations

Mar 10, 2000·Biochemical and Biophysical Research Communications·K S LeeB L Wanner
Jan 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K K Ericson, T J Yang
Aug 24, 2004·Applied Microbiology and Biotechnology·Evi Andronopoulou, Constantinos E Vorgias
Oct 20, 2005·Journal of Bioscience and Bioengineering·Ratchaneewan Aunpad, Watanalai Panbangred
Aug 15, 1991·European Journal of Biochemistry·R WetzkerM Waterfield
Jun 15, 1992·European Journal of Biochemistry·J de la CruzA Llobell
Apr 2, 2004·The Journal of General and Applied Microbiology·Duo-Chuan LiJing Lu
Apr 10, 2015·European Journal of Protistology·Erhan Aslan, Muhittin Arslanyolu
Jun 17, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I GálG Szabó
Dec 18, 1998·The Journal of Biological Chemistry·A UrbanoF Foss
Jun 9, 2006·Mycopathologia·Li Duo-Chuan
Mar 2, 1999·Applied and Environmental Microbiology·C CarsolioA Herrera-Estrella
May 1, 1995·Applied and Environmental Microbiology·L CherninI Chet
Dec 1, 1995·Infection and Immunity·G M Escott, D J Adams
Dec 1, 1993·Journal of Bacteriology·F C MinionE Tigges
May 15, 1992·Analytical Biochemistry·O Gabriel, D M Gersten

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.