Enhanced responses to a DNA vaccine encoding a fusion antigen that is directed to sites of immune induction

Nature
J S BoyleAndrew M Lew

Abstract

Viral infection and vaccination with DNA both induce similar immune responses to encoded antigens that are produced by the host. The availability of antigens in lymphoid organs is important in generating an immune response to viral challenge. Antigen availability may also be important in the response to DNA vaccines, because immune responses are stronger when antigen is secreted from DNA-transfected cells. We directed antigen to lymphoid organs by vaccination with DNA encoding antigen-ligand fusion proteins. The two ligands examined bind to receptors that are present on high endothelial venule cells of lymph nodes or on antigen-presenting cells. Here we show that both the humoral and the cellular immune responses to a model DNA vaccine were enhanced using either antigen-targeting strategy. Moreover, directing antigen to antigen-presenting cells speeded up, and altered the form of, the immune response. Directing antigen to sites of immune-response induction may represent a generic means of tailoring a potent and effective immune response to a DNA vaccine.

References

Mar 23, 1990·Science·J A WolffP L Felgner
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P S LinsleyJ A Ledbetter
Sep 14, 1990·Journal of Immunological Methods·Y Aida, M J Pabst
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·M L MichelR G Whalen
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·E RazM A Yankauckas
Jan 1, 1993·Immunology Today·C M Snapper, J J Mond
Dec 1, 1996·The Journal of Experimental Medicine·M D CatalinaM H Siegelman
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·J S BoyleA M Lew

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Citations

Jun 22, 1999·Immunologic Research·E R Brandt, M F Good
Apr 14, 2006·Cancer Immunology, Immunotherapy : CII·Dhanalakshmi ChinnasamyMartin K Oaks
Mar 21, 2001·Vaccine·J Y Scheerlinck
Sep 27, 2003·Methods : a Companion to Methods in Enzymology·Nicole A Doria-Rose, Nancy L Haigwood
May 14, 1999·Pharmaceutical Science & Technology Today·K E ShroffT J Higgins
May 19, 1998·Nature Medicine·S Langermann
May 11, 2002·Nature Reviews. Immunology·Drew M Pardoll
Jul 5, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Chang-Yao HsiehWen-Fang Cheng
Mar 29, 2000·Scandinavian Journal of Immunology·C SvanholmM E Rottenberg
Aug 18, 2000·Immunology and Cell Biology·C Demangel, W J Britton
Mar 21, 2002·Immunology and Cell Biology·A K Sharma, G K Khuller
Jun 14, 2001·DNA and Cell Biology·A CanoK Manoutcharian
Mar 6, 2004·DNA and Cell Biology·Toshi NagataYukio Koide
Dec 3, 1999·Immunological Reviews·A J RamsayI A Ramshaw
Dec 3, 1999·Immunological Reviews·R M MaizelsM Yazdanbakhsh
Jul 21, 2007·Acta Pharmacologica Sinica·Feng ZhangQi-wei Tian
Dec 13, 2005·Clinical and Diagnostic Laboratory Immunology·Yanwen JinQingjun Ma
Jun 3, 2000·Annual Review of Immunology·S GurunathanR A Seder
Mar 8, 2008·The Journal of Clinical Investigation·Godwin NchindaRalph M Steinman
Dec 30, 2008·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Michelle R DavisWilliam Van Bonn
Jul 15, 1999·Clinical and Experimental Immunology·R LaylorT Lund
Nov 28, 2002·Clinical and Experimental Immunology·L BandholtzM E Rottenberg
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·K R TrenholmeD J Kemp

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