Abstract
We previously demonstrated stimulation of collateral vessel formation in a rabbit model of unilateral limb ischaemia after administration of endothelial cell growth factor (ECGF). To distinguish clearly the effects of ischaemia alone from those of ischaemia combined with angiogenic stimulation in the same animal, a model of bilateral hindlimb ischaemia was used to evaluate further the angiogenic effect of ECGF. Ischaemia was produced in both hindlimbs of 11 rabbits by femoral artery excision. Beginning 10 days later, ECGF (8 mg in 3 ml of saline) was injected in one hindlimb while 3 ml of saline alone was injected in the other every other day for a total of five doses. Calf systolic blood pressure was measured in both limbs on postoperative days, 10, 30, and 50. On day 50, collateral formation was quantitated angiographically, and muscle samples were obtained for quantitation of capillary density and histologic studies. The mean calf systolic blood pressure in the both hindlimbs was similar on day 10 (36.9 +/- 2.3 versus 38.1 +/- 2.9 mmHg) but was significantly higher in the ECGF-treated limb on day 30 (68.9 +/- 3.1 versus 45.0 +/- 2.9 mmHg) and day 50 (83.0 +/- 3.0 versus 57.0 +/- 1.7; p < 0.0001 for both comparisons). On day ...Continue Reading
References
Sep 4, 1992·Science·A Yanagisawa-MiwaC Kurashima
Jul 1, 1991·Circulation Research·S BanaiE F Unger
Aug 1, 1991·The Annals of Thoracic Surgery·V M OlechG A Patterson
Oct 1, 1991·Annals of Surgery·J FolkmanY Shing
Sep 6, 1991·Brain Research·M K LyonsF B Meyer
Feb 1, 1990·Annals of Surgery·D P MooneyR L Gamelli
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A B SchreiberT Maciag
Jan 1, 1987·Annual Review of Physiology·P A D'Amore, R W Thompson
Jan 1, 1987·Nouvelle Revue Française D'hématologie·A Camez, G Tobelem
May 1, 1988·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·B L EppleyJ Feder
Jun 1, 1989·Archives of Surgery·M Höckel, J F Burke
Oct 1, 1987·Seminars in Thrombosis and Hemostasis·J Joseph-Silverstein, D B Rifkin
Mar 13, 1986·Biochemical and Biophysical Research Communications·G Gimenez-GallegoK A Thomas
Nov 11, 1983·Science·S C ThorntonE M Levine
Jun 1, 1993·The Journal of Surgical Research·L Q PuJ F Symes
Jul 1, 1993·Circulation·L Q PuJ F Symes
Citations
Apr 10, 2013·Gene·Anna Grochot-PrzeczekAlicja Jozkowicz
Jun 6, 2012·Acta Cirúrgica Brasileira·Victor Araujo FelzemburghJosé Humberto Oliveira Campos
Feb 13, 2007·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·J M LassoR Pérez Cano
Jul 28, 2001·European Journal of Clinical Investigation·T T RissanenS Ylä-Herttuala
May 8, 2018·Neurosurgical Review·Ali Tayebi Meybodi, Michael T Lawton
Mar 3, 2004·Vascular Medicine·Valeri S ChekanovMasood Akhtar
Jul 16, 2004·Physiological Measurement·Oleg Y GrinbergHarold M Swartz
Mar 30, 2000·British Journal of Plastic Surgery·Z KrygerH J Buncke