Enhanced (S)-linalool production by fusion expression of farnesyl diphosphate synthase and linalool synthase in Saccharomyces cerevisiae

Journal of Applied Microbiology
Yu DengJingwen Zhou

Abstract

In order to improve the availability of geranyl diphosphate (GPP) in the mevalonate pathway for enhancing (S)-linalool production in Saccharomyces cerevisiae. A (S)-linalool synthase (LIS): AaLS1 from Actinidia arguta was co-expressed with FPPS with different peptide linkers to redirect the flux from geranyl diphosphate (GPP) to (S)-linalool production in S. cerevisiae. The strain with the best peptide linker((GGGGS)3 ), produced 101.55±2.97μg l(-1) (S)-linalool, a 69.7% increase compared to those with two independent LIS and FPPS expressed. In a 3-L fermenter, the (S)-linalool titer was further improved to 240.64±5.31μg l(-1) . The results demonstrate that the fusion proteins catalyzing consecutive steps in a metabolic pathway significantly improved the (S)-linalool production with GPP as precursor. The fusion protein strategy co-expressing AaLS1 and FPPS, assembled with a long peptide linker made S. cerevisiae produced the highest reported (S)-Linalool titer to date. This article is protected by copyright. All rights reserved.

References

Jun 2, 2000·Protein Engineering·C J Crasto, J A Feng
Aug 9, 2002·Trends in Plant Science·Soheil S Mahmoud, Rodney B Croteau
Jan 23, 2003·Protein Engineering·Richard A George, Jaap Heringa
Apr 30, 2005·Current Opinion in Plant Biology·Kirsten JørgensenBirger Lindberg Møller
Nov 14, 2006·FEMS Yeast Research·Marilyne OswaldFrancis Karst
Nov 23, 2006·Applied Microbiology and Biotechnology·Sydnor T Withers, Jay D Keasling
May 24, 2007·Applied Microbiology and Biotechnology·Izumi OritaYasuyoshi Sakai
Jul 3, 2007·Applied and Environmental Microbiology·Juan ZhangJian Chen
Nov 13, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F BakkaliM Idaomar
Apr 9, 2008·Current Opinion in Biotechnology·Natalia Dudareva, Eran Pichersky
Apr 17, 2008·Applied Microbiology and Biotechnology·Ping Lu, Ming-Guang Feng
Aug 30, 2008·Current Opinion in Biotechnology·Robert J ConradoMatthew P DeLisa
Sep 9, 2008·Drug Discovery Today·Sang Yup LeeTae Yong Kim
Jul 7, 2009·Annual Review of Plant Biology·James Kirby, Jay D Keasling
Jul 14, 2009·Applied and Environmental Microbiology·Kenro TokuhiroSakayu Shimizu
Aug 12, 2009·Applied Microbiology and Biotechnology·Remco MuntendamOliver Kayser
Dec 15, 2010·Applied and Environmental Microbiology·Line AlbertsenUffe H Mortensen
Feb 12, 2011·Current Opinion in Biotechnology·Norihiko Misawa
Mar 11, 2011·Biotechnology and Bioengineering·Marc J C FischerFrancis Karst
May 24, 2011·Metabolic Engineering·Moran FarhiAlexander Vainstein
Dec 2, 2011·Journal of the American Chemical Society·Yechun WangJoseph M Jez
Dec 6, 2011·FEMS Yeast Research·Michael S SiddiquiChristina D Smolke
Jul 10, 2012·Journal of Agricultural and Food Chemistry·Bing-Ho ChengShang-Tzen Chang

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Citations

Jun 5, 2020·Journal of Industrial Microbiology & Biotechnology·Zhihui HuDongguang Xiao
Oct 6, 2020·Frontiers in Bioengineering and Biotechnology·Tianqing SongYingjin Yuan
Nov 4, 2020·Microbial Cell Factories·Behnaz NowrouziLeonardo Rios-Solis
Jan 5, 2021·Applied Microbiology and Biotechnology·Govinda R NavaleSandip S Shinde
Mar 4, 2021·Microbial Cell Factories·Nobuhisa NittaYoshihiro Usuda
May 14, 2021·Journal of Agricultural and Food Chemistry·Jing WuXun Li
May 4, 2021·Chembiochem : a European Journal of Chemical Biology·Clara A FerrazNigel S Scrutton

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