Enhanced Salt Tolerance Conferred by the Complete 2.3 kb cDNA of the Rice Vacuolar Na(+)/H(+) Antiporter Gene Compared to 1.9 kb Coding Region with 5' UTR in Transgenic Lines of Rice

Frontiers in Plant Science
U S M AminZeba I Seraj

Abstract

Soil salinity is one of the most challenging problems that restricts the normal growth and production of rice worldwide. It has therefore become very important to produce more saline tolerant rice varieties. This study shows constitutive over-expression of the vacuolar Na(+)/H(+) antiporter gene (OsNHX1) from the rice landrace (Pokkali) and attainment of enhanced level of salinity tolerance in transgenic rice plants. It also shows that inclusion of the complete un-translated regions (UTRs) of the alternatively spliced OsNHX1 gene provides a higher level of tolerance to the transgenic rice. Two separate transformation events of the OsNHX1 gene, one with 1.9 kb region containing the 5' UTR with CDS and the other of 2.3 kb, including 5' UTR, CDS, and the 3' UTR regions were performed. The transgenic plants with these two different constructs were advanced to the T3 generation and physiological and molecular screening of homozygous plants was conducted at seedling and reproductive stages under salinity (NaCl) stress. Both transgenic lines were observed to be tolerant compared to WT plants at both physiological stages. However, the transgenic lines containing the CDS with both the 5' and 3' UTR were significantly more tolerant compa...Continue Reading

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Citations

Nov 22, 2019·Frontiers in Plant Science·Mohammad Umer Sharif ShohanZeba I Seraj
Aug 5, 2017·Frontiers in Physiology·Dekoum V M AssahaMahmoud W Yaish
Mar 7, 2021·International Journal of Molecular Sciences·Kimberly S PonceGuoyou Ye

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Datasets Mentioned

BETA
AB021878

Methods Mentioned

BETA
environmental stress
transgenic
PCR
phosphotransferase
transgenics
salt stress

Software Mentioned

ToolPak
Excel

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