Enhanced SARS-CoV-2 Neutralization by Secretory IgA in vitro

BioRxiv : the Preprint Server for Biology
Z. WangMichel C Nussenzweig

Abstract

SARS-CoV-2 primarily infects cells at mucosal surfaces. Serum neutralizing antibody responses are variable and generally low in individuals that suffer mild forms of the illness. Although potent IgG antibodies can neutralize the virus, less is known about secretory antibodies such as IgA that might impact the initial viral spread and transmissibility from the mucosa. Here we characterize the IgA response to SARS-CoV-2 in a cohort of 149 individuals. IgA responses in plasma generally correlate with IgG responses and clones of IgM, IgG and IgA producing B cells that are derived from common progenitors are evident. Plasma IgA monomers are 2-fold less potent than IgG equivalents. However, IgA dimers, the primary form in the nasopharynx, are on average 15 times more potent than IgA monomers. Thus, secretory IgA responses may be particularly valuable for protection against SARS-CoV-2 and for vaccine efficacy.

Methods Mentioned

BETA
ELISA
ELISAs
flow cytometry
PCR
biolayer interferometry
size exclusion chromatography

Related Concepts

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

BioRxiv : the Preprint Server for Biology
Zijun WangMichel C Nussenzweig
Science Translational Medicine
Zijun WangMichel C Nussenzweig
Proceedings of the National Academy of Sciences of the United States of America
M B MazanecJ G Nedrud
The Journal of Immunology : Official Journal of the American Association of Immunologists
T A Platts-Mills, K Ishizaka
© 2021 Meta ULC. All rights reserved