Enhanced secretion and activation of matrilysin during malignant conversion of human colorectal epithelium and its relationship with invasive potential of colon cancer cells

Cancer
F ItohK Imai

Abstract

Matrilysin is a member of the matrix metalloproteinase gene family which is believed to play an important role in tumor progression. Matrilysin mRNA has been reported to be overexpressed in colorectal cancer. The aim of this study was to evaluate the enzyme activity of this protein during colorectal cancer. The aim of this study was to evaluate the enzyme activity of this protein during colorectal carcinogenesis and its relationship with the invasiveness of colorectal cancer cells. We have examined the levels of secreted matrilysin in various epithelial disorders of the colon using casein zymography. We have also examined the effect of matrilysin on the in vitro invasiveness of colorectal cancer cells using a modified Boyden Chamber method. The enzyme activities of matrilysin were detected in cancer tissue and adenoma tissue, whereas they were hardly detectable in hyperplastic polyps, mildly inflamed regions of ulcerative colitis, and normal colon tissue. Enhanced secretion and enhanced activation of matrilysin were observed in cancer. An in vitro invasion assay revealed that the levels of secreted matrilysin-transfectants correlated positively with invasiveness. Our data suggest that the secretion and activation of matrilysin ...Continue Reading

References

Nov 11, 1991·International Journal of Cancer. Journal International Du Cancer·R E HewittD R Turner
Apr 1, 1990·Trends in Genetics : TIG·L M Matrisian
May 23, 1994·FEBS Letters·T CrabbeA Docherty
Feb 1, 1994·Biochemical Society Transactions·M I CockettA J Docherty
Feb 1, 1994·Biochemical Society Transactions·S McDonnellL M Matrisian
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·H Birkedal-HansenJ A Engler
Jun 19, 1993·International Journal of Cancer. Journal International Du Cancer·M YoshimotoA Yachi

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Citations

Aug 6, 2009·The Journal of Biological Chemistry·Hyun-Jeong RaWilliam C Parks
Nov 27, 2002·Clinical Colorectal Cancer·Angela G Mysliwiec, Deborah L Ornstein
Nov 5, 1999·The Journal of Pathology·S Curran, G I Murray
Aug 9, 2001·International Journal of Cancer. Journal International Du Cancer·Y AdachiK Imai
Jan 19, 2005·World Journal of Gastroenterology : WJG·Li LiuYan-Qing Ding
May 1, 2009·Nature Reviews. Gastroenterology & Hepatology·Linda A FeaginsStuart J Spechler
Jun 9, 2001·Seminars in Surgical Oncology·E SeregniE Bombardieri
Nov 4, 2005·Breast Cancer Research and Treatment·Karolina PrzybylowskaJanusz Blasiak
Jul 31, 2008·International Journal of Colorectal Disease·Xi-Ling ZhuYan-Qing Ding
Sep 19, 2006·BJU International·Wei-Min YangDong-Sheng Guo
Jul 12, 2002·Molecular Carcinogenesis·Ken J NewellDavid K Driman
Feb 2, 2011·The American Journal of Pathology·Robert TellRichard V Benya
Jan 10, 2002·Gastroenterology·Andrei V OugolkovToshinari Minamoto

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