PMID: 9004540Jan 1, 1996Paper

Enhanced secretory ability for the human factor VIII light chain produced in baculovirus-infected insect cells

Cytotechnology
E WebbI Nisbet

Abstract

The light and truncated heavy chains of human factor VIII, expressed separately in baculovirus-infected insect cells, exhibited different secretory behaviour when compared with each other and with a biologically active fusion molecule of the truncated heavy and light chains. The light chain was very efficiently secreted into culture medium, as judged by high extracellular protein levels and the absence of evidence for light chain retention within cells. Alternatively, proteins containing the heavy chain sequence were poorly secreted and appeared to be sequestered within cells, suggesting that regions within the heavy chain are responsible for the low levels of secreted protein which have generally been observed for recombinant factor VIII.

References

Oct 24, 1991·Nature·G C FlynnJ E Rothman
Nov 9, 1989·Nature·R J Kaufman
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J J TooleR J Kaufman
Jan 29, 1993·Biochemical and Biophysical Research Communications·E WebbI Nisbet
Apr 1, 1993·Virology·R W DomsA Helenius

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Citations

May 27, 2011·Thrombosis and Haemostasis·Jean-Luc PellequerJean-Luc Plantier

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