Enhanced sensitivity of Arabidopsis anthocyanin mutants to photooxidation: a study with fluorescence imaging

Functional Plant Biology : FPB
Ling ShaoQun Gu

Abstract

Chlorophyll fluorescence imaging and antioxidative capability in detached leaves of the wild-type Arabidopsis thaliana ecotype Landsberg erecta (Ler) and in three mutants deficient in anthocyanin biosynthesis (tt3, tt4 and tt3tt4) were investigated under photooxidation stress induced by methyl viologen (5 μm) in the light. In comparison with the wild-type (WT) plant, photooxidation resulted in significant decreases in the contents of total phenolics and flavonoid, total antioxidative capability and chlorophyll fluorescence parameters (Fv/Fm, qP, ΦPSII, NPQ and ETR) as determined by chlorophyll fluorescence imaging, and in an increase in cell-membrane leakiness in the three anthocyanin mutants. The sequence of sensitivity to photooxidation in the leaves of the four phenotypes were tt3tt4 (deficient in both chalcone synthase locus (CHS) and dihydroflavonol 4-reductase locus (DFR)) > tt4 (deficient in CHS) > tt3 (deficient in DFR) > WT. The results demonstrate that anthocyanins might, along with other antioxidants, protect the photosynthetic apparatus against photooxidative damage. An interesting phenomenon was observed over the 270 min of the photooxidative treatment, that is, fluorescence imaging revealed that qP, ΦPSII and ETR ...Continue Reading

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Citations

Dec 2, 2009·Physiologia Plantarum·Xue-Qin ZengChang-Lian Peng
Mar 7, 2020·The Plant Journal : for Cell and Molecular Biology·Davide MarziMaura Cardarelli
Jul 6, 2014·Oecologia·H M Appel, R B Cocroft
Jun 11, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Laming PeiHui Li

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