Enhanced steroidogenic and altered antioxidant response by ZnO nanoparticles in mouse testis Leydig cells

Toxicology and Industrial Health
Nisha Bara, Gautam Kaul

Abstract

Zinc oxide nanoparticles (ZnO NPs) are important nanomaterials with myriad applications and in widespread use. The main aim of this study was to evaluate the direct effect of ZnO NPs on steroidogenesis by considering mouse testicular Leydig cells (TM3) as an in vitro model system. The uptake, intracellular behaviour, cytotoxicity and morphological changes induced by ZnO NPs (0-200 µg/ml) in a time-dependent manner in the TM3 were assessed. A significant ( p < 0.05) decrease in TM3 viability was observed at 2 µg/ml ZnO NP after a 1-h incubation time period. Increased antioxidant enzyme activity, namely, superoxide dismutase (SOD) and catalase, was regularly observed. Not surprisingly, apoptosis also increased significantly after a 4-h exposure period. Transmission electron micrographs illustrated that ZnO NPs were taken up by Leydig cells and resulted in the formation of autophagosomes, autolysosomes and autophagic vacuoles. Concomitant real-time data indicated that ZnO NPs significantly increased the expression of steroidogenesis-related genes (steroidogenic acute regulatory protein and cytochrome P450 side-chain cleavage enzyme) and significantly ( p < 0.05) decreased antioxidant enzyme gene (SOD) expression after a 4-h incuba...Continue Reading

References

Feb 1, 1985·Toxicology Letters·E Borenfreund, J A Puerner
Jan 1, 1984·Methods in Enzymology·H Aebi
Feb 9, 1996·Biochemical Pharmacology·D M Stocco, B J Clark
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Jan 13, 2006·American Journal of Physiology. Cell Physiology·Hideyuki Yamawaki, Naoharu Iwai
Sep 19, 2006·The International Journal of Biochemistry & Cell Biology·Marian ValkoJoshua Telser
Feb 7, 2007·The Journal of Endocrinology·Palaniappan MurugesanJagadeesan Arunakaran
Mar 14, 2007·Autophagy·Olga ZabirnykOleksandr Seleverstov
May 19, 2007·Genes & Development·Robin MathewEileen White
Jan 26, 2008·International Journal of Andrology·S YoshidaT Shibamoto
Feb 29, 2008·Nature·Noboru MizushimaDaniel J Klionsky
Jul 5, 2008·Nature Protocols·Guillermo RepettoJorge L Zurita
Sep 23, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Tomoko KomatsuKen Takeda
Oct 18, 2008·Progress in Neurobiology·Won Hyuk SuhYoo-Hun Suh
Oct 31, 2008·Nature Reviews. Molecular Cell Biology·Guido Kroemer, Beth Levine
Nov 6, 2008·Toxicology and Applied Pharmacology·N A Monteiro-RiviereL W Zhang
Oct 1, 2009·The Journal of Reproduction and Development·Rosemary Stelzer, Reinhold J Hutz
Jan 7, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Peter Schlattmann, Ulrich Dirnagl
May 4, 2010·Toxicology and Applied Pharmacology·Barbara De BerardisStefania Meschini
May 15, 2010·Biomaterials·Jasmine J LiLin-Yue L Yung
Mar 3, 2011·Journal of Biomedical Nanotechnology·Towseef Rafeeqi, Gautam Kaul
Apr 9, 2011·Antioxidants & Redox Signaling·Tohru Fukai, Masuko Ushio-Fukai
Jun 7, 2011·The International Journal of Biochemistry & Cell Biology·Shu-ya WangBo Liu
Apr 25, 2012·Annual Review of Biomedical Engineering·Alexandre AlbaneseWarren C W Chan
Mar 6, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Kyeong-Nam YuMyung-Haing Cho
Mar 29, 2013·Environment International·Vanessa ValdiglesiasJoão Paulo Teixeira
May 1, 2013·International Journal of Molecular Sciences·Jianling SunBing Yan
Aug 21, 2013·Journal of Assisted Reproduction and Genetics·Ali Reza TalebiMahnaz Moridian
Jun 27, 2014·Nanotechnology, Science and Applications·Bhupinder Singh Sekhon
Jun 2, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Qian LiuQunwei Zhang
Jul 12, 2017·Analytical Biochemistry·Andrew OsahorKumaran Narayanan

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Citations

Aug 23, 2019·International Journal of Molecular Sciences·Jingcao ShenJiaxiang Chen
Jul 5, 2019·Environmental Science and Pollution Research International·Zeinab RafieeFereshteh Nejad-Dehbashi
Mar 20, 2021·The Science of the Total Environment·Maingredy Rodrigues SouzaThiago Lopes Rocha
Aug 8, 2021·International Journal of Molecular Sciences·Mariana VassalMaria de Lourdes Pereira
Dec 23, 2021·Journal of Applied Toxicology : JAT·Yongshuai Yao, Meng Tang
Aug 11, 2020·The International Journal of Biochemistry & Cell Biology·Azam JavadiFereshteh Esfandiari

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Methods Mentioned

BETA
dynamic
scanning
transmission electron microscopy
scraping
PCR
ELISA
transmission
scanning electron microscopy

Software Mentioned

GraphPad Prism
GraphPad

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