Enhanced stimulation of Akt-3/protein kinase B-gamma in human aortic smooth muscle cells

Biochemical and Biophysical Research Communications
Lakshman Sandirasegarane, Mark Kester

Abstract

Growth factor-induced activation of Akt (protein kinase B) is implicated in the proliferation of vascular smooth muscle cells (VSMC) in addition to antiapoptotic signaling. Although previous studies have documented increases in total Akt or Akt-1 activity in rodent VSMC, little is known about the regulation of Akt-2 or Akt-3 kinase activity in VSMC from any species. In the present study, reverse transcriptase-polymerase chain reaction revealed the expression of all three Akt isoforms in human aortic VSMC. In vitro kinase assays using immunoprecipitated Akt isoforms showed robust increases in Akt-3 activity after stimulation of human aortic VSMC with platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor-1. In contrast, these growth factors produced modest and marginal increases in Akt-1 and Akt-2 kinase activity, respectively. Pretreatment of VSMC with a phosphoinositide-3kinase (PI-3K) inhibitor, LY294002, led to significant inhibition of growth factor(s)-induced increases in Akt-3 activity and DNA synthesis. The present findings provide the first direct evidence that the Akt-3 isoform is predominantly activated in human aortic VSMC. Moreover, these data suggest that PI-3K-dependent activation of Akt-3 ...Continue Reading

References

Mar 1, 1996·Journal of the American College of Cardiology·D AronsonE J Rayfield
Dec 31, 1997·The Journal of Biological Chemistry·R MeierB A Hemmings
Apr 29, 1998·The Journal of Biological Chemistry·M R CaleraP F Pilch
Aug 17, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·B MeisterR Möhle
Sep 22, 1999·European Journal of Biochemistry·S MasureA Richardson
Jul 26, 2000·The Journal of Biological Chemistry·J OkanoH Nakagawa

❮ Previous
Next ❯

Citations

Mar 29, 2011·Human Molecular Genetics·Michael A GrassiDan L Nicolae
Jan 6, 2011·American Journal of Physiology. Endocrinology and Metabolism·Todd E FoxThomas W Gardner
Apr 2, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Min DingKathleen A Martin
Aug 30, 2014·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Sami Alkayyali, Valeriya Lyssenko
Mar 19, 2014·PloS One·Hui YuChristopher A Bidwell
Apr 6, 2013·Journal of Diabetes and Its Complications·Cecilia C Low WangGregory G Schwartz
Nov 15, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·L BergandiA Bosia
Mar 6, 2008·Journal of Vascular Research·Nobutake SadamasaKikuya Kato
Feb 20, 2007·The Journal of Biological Chemistry·Todd E FoxMark Kester
Mar 31, 2019·British Journal of Pharmacology·Qin FangDao Wen Wang
Jul 23, 2015·American Journal of Physiology. Endocrinology and Metabolism·Thomas W GardnerPatrice E Fort
Jul 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Elena A GoncharovaVera P Krymskaya
Nov 28, 2001·The Journal of Biological Chemistry·Nicole A BourbonMark Kester
Jan 16, 2003·American Journal of Physiology. Endocrinology and Metabolism·Gail A WongRobert H Weiss
Apr 30, 2017·Clinical Epigenetics·Bénédicte L TremblayMarie-Claude Vohl
Sep 5, 2019·NPJ Genomic Medicine·Chang LiuCharles Rotimi
Jun 12, 2003·American Journal of Physiology. Endocrinology and Metabolism·Chad E N ReiterThomas W Gardner

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Related Papers

BMJ : British Medical Journal
Pierfrancesco Agostoni, Frank Van den Branden
Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology
Matthias D Hofer, Mark A Rubin
American Journal of Physiology. Endocrinology and Metabolism
Todd E FoxThomas W Gardner
© 2021 Meta ULC. All rights reserved