Enhanced suppressor of cytokine signaling 3 in arthritic cartilage dysregulates human chondrocyte function

Arthritis and Rheumatism
F A J van de LooW B van den Berg

Abstract

To determine the expression of suppressor of cytokine signaling 3 (SOCS-3) in human articular chondrocytes and its functional consequences. Chondrocytes were isolated from the cartilage of patients with osteoarthritis (OA), patients with rheumatoid arthritis (RA), and trauma patients and from the healthy cartilage of patients with a femoral neck fracture. The human chondrocyte cell line G6 and primary bovine chondrocytes were used in validation experiments. SOCS-3 messenger RNA (mRNA) expression was measured by quantitative polymerase chain reaction, and SOCS-3 protein levels were determined by Western blotting and immunohistochemical analysis. To ascertain the role of SOCS-3 in the chondrocyte response to interleukin-1β (IL-1β) or lipopolysaccharide (LPS), the expression of SOCS3 was either reduced by small interfering RNA or enhanced by viral transduction. The expression of SOCS-3 mRNA (but not that of SOCS-1 mRNA) was significantly enhanced in chondrocytes obtained from OA cartilage (mean ± SD ΔC(t) 3.4 ± 1.0) and RA cartilage (ΔC(t) 3.4 ± 1.4) compared with cartilage obtained from patients with femoral neck fracture (ΔC(t) 5.3 ± 1.2). The expression of SOCS3 correlated significantly with that of other genes known to be expr...Continue Reading

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Citations

Aug 25, 2015·Drug Discovery Today·Yu-Hsiang Hsu, Ming-Shi Chang
Apr 13, 2015·Current Opinion in Pharmacology·Mary B Goldring, Francis Berenbaum
Mar 31, 2015·International Journal of Inflammation·Marta ImamuraLinamara Rizzo Battistella
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