PMID: 3762561Sep 1, 1986Paper

Enhanced survival by photoreactivation and liquid holding following UV damage of TN-368 insect cells

Mutation Research
T M Koval

Abstract

These studies demonstrate that the TN-368 lepidopteran insect cell line, which is extremely resistant to the lethal effects of ionizing radiation, is also quite resistant to 254-nm ultraviolet light. While resistance to ionizing radiation in TN-368 cells has been associated with superior DNA repair processes, previous findings have indicated no correlation between survival ability and amount of unscheduled DNA synthesis in response to ultraviolet light. The present studies were undertaken to define the TN-368 ultraviolet light survival response, the ability of the cells to repair UV-induced damage by photoreactivation, the capacity of the cells to undergo UV repair during liquid holding in the dark, and the relationship between photoreactivation and liquid-holding recovery. Survival was assayed by colony formation. 254-nm irradiations were performed using germicidal lamps and photoreactivation was accomplished using black lights. Photoreactivable sectors of UV damage at 50 and 10% survival are 0.65 and 0.68, respectively. Survival responses, both with and without photoreactivation, have a small initial shoulder followed by an exponential region, and finally the curves continue to decrease but with decreasing slope. F0, Fq, and ...Continue Reading

References

Aug 1, 1980·Photochemistry and Photobiology·D Roczen, H D Mennigmann
Jul 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·R B SETLOW, J K SETLOW
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T M Koval

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Citations

Nov 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·T M Koval
Jun 11, 2011·The International Journal of Biochemistry & Cell Biology·Shubhankar SumanSudhir Chandna
Mar 7, 2009·Mutagenesis·I-Cheng ChengT C Wang

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