PMID: 8592684Nov 1, 1995Paper

Enhanced therapeutic effect of cytidine-5'-diphosphate choline when associated with GM1 containing small liposomes as demonstrated in a rat ischemia model

Pharmaceutical Research
M FrestaG Puglisi

Abstract

Cytidine-5'-diphosphate choline (CDPc) was encapsulated in long-circulating unilamellar vesicles (SUVs) to improve the drug's biological effectiveness. SUVs made up of diaplmitoylphosphatidylcholine/diaplmitoylphosphatidylserine /cholesterol (7:4:7 molar ratio) and 8 mol % of ganglioside GM1 were prepared by extrusion through polycarbonate filters (mean diameter 50 nm). The formulation effectiveness was evaluated by an in vivo model of cerebral ischemia on Wistar rats. The enhanced delivery of CDPc into the brain improved the therapeutic effectiveness of the drug. CDPc-loaded SUVs improved the survival rate of ischemized and reperfused Wistar rats (320-350 g) by approximately 66% compared with the free drug. Liposome formulation was also able to effectively protect the brain against peroxidative damage caused by post-ischemic reperfusion. SUVs lowered the conjugated diene levels of the cerebral cortex. The liposomal delivery system did not alter the distribution patterns in the various cerebral lipid fractions of the drug, radiolabeled with 14C-CDPc. CDPc-loaded SUVs were able to protect the brain against damage induced by ischemia. A possible clinical application is envisaged.

Citations

Aug 22, 2000·Antimicrobial Agents and Chemotherapy·P M FurneriG Tempera
Feb 23, 2018·Expert Opinion on Drug Delivery·Zahraa S Al-Ahmady
Nov 27, 2002·Clinical and Experimental Hypertension : CHE·Francesco AmentaDaniele Tomassoni
Sep 25, 2002·Journal of Neuroscience Research·Rao Muralikrishna Adibhatla, J F Hatcher
Feb 18, 2010·Medicinal Research Reviews·Christian CeliaMassimo Fresta
Jan 30, 2013·Nature Reviews. Neurology·Rao Muralikrishna Adibhatla
Apr 11, 2006·Journal of Drug Targeting·Jannapally Suresh ReddyGerben A Koning
Jul 20, 1999·The Journal of Pharmacy and Pharmacology·M FrestaG Puglisi
Feb 2, 2018·The Journal of Physical Chemistry Letters·Bong Jin HongSonBinh T Nguyen
Nov 17, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·M J RosemaryT Pradeep

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Related Papers

Journal of B.U.ON. : Official Journal of the Balkan Union of Oncology
O KanatMine Sibel Gurun
Pharmaceutical Research
Didier HoarauJean-Christophe Leroux
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
Bing-jiang ZhangXiao-ping Liu
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
Wei HongHai-Yang Hu
© 2021 Meta ULC. All rights reserved