Enhanced tolerance of Saccharomyces cerevisiae to multiple lignocellulose-derived inhibitors through modulation of spermidine contents

Metabolic Engineering
Sun-Ki KimJin-Ho Seo

Abstract

Fermentation inhibitors present in lignocellulose hydrolysates are inevitable obstacles for achieving economic production of biofuels and biochemicals by industrial microorganisms. Here we show that spermidine (SPD) functions as a chemical elicitor for enhanced tolerance of Saccharomyces cerevisiae against major fermentation inhibitors. In addition, the feasibility of constructing an engineered S. cerevisiae strain capable of tolerating toxic levels of the major inhibitors without exogenous addition of SPD was explored. Specifically, we altered expression levels of the genes in the SPD biosynthetic pathway. Also, OAZ1 coding for ornithine decarboxylase (ODC) antizyme and TPO1 coding for the polyamine transport protein were disrupted to increase intracellular SPD levels through alleviation of feedback inhibition on ODC and prevention of SPD excretion, respectively. Especially, the strain with combination of OAZ1 and TPO1 double disruption and overexpression of SPE3 not only contained spermidine content of 1.1mg SPD/g cell, which was 171% higher than that of the control strain, but also exhibited 60% and 33% shorter lag-phase period than that of the control strain under the medium containing furan derivatives and acetic acid, res...Continue Reading

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Citations

Jan 10, 2016·Current Opinion in Biotechnology·Yun Chen, Jens Nielsen
Sep 1, 2015·Current Opinion in Biotechnology·Zongjie Dai, Jens Nielsen
Dec 1, 2015·Frontiers in Bioengineering and Biotechnology·Luis CaspetaJens Nielsen
Oct 29, 2017·World Journal of Microbiology & Biotechnology·Aslıhan Örs Gevrekci
Jan 26, 2017·Bioprocess and Biosystems Engineering·Sun-Ki KimJin-Ho Seo
Apr 14, 2017·World Journal of Microbiology & Biotechnology·Peng GengGui Yang Shi
Aug 14, 2019·Nature Communications·Prashanth Srinivasan, Christina D Smolke
Dec 10, 2020·Biotechnology for Biofuels·Li WangYing-Jin Yuan
Feb 27, 2018·Metabolic Engineering·Rongming LiuRyan T Gill
Mar 21, 2021·Applied Microbiology and Biotechnology·Lahiru N Jayakody, Yong-Su Jin

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