Enhanced treatment of articular cartilage defect of the knee by intra-articular injection of Bcl-xL-engineered mesenchymal stem cells in rabbit model

Journal of Tissue Engineering and Regenerative Medicine
Bin HuTian-Qiu Mao

Abstract

Direct intra-articular injection of mesenchymal stem cells (MSCs) has been proposed as a potential cell therapy for cartilage defects. This cell therapy relies on the survival of the implanted MSCs. However, the arduous local environment may limit cell viability after implantation, which would restrict the cells' regenerative capacity. Thus, it is necessary to reinforce the implanted cells against the unfavourable microenvironment in order to improve the efficacy of cell therapy. We examined whether the transduction of an anti-apoptotic protein, Bcl-xL, into MSCs could prevent cell death and improve the implantation efficiency of MSCs in a rabbit model. Our current findings demonstrate that the group treated with Bcl-xL-engineered MSCs could improve cartilage healing both morphologically and histologically when compared with the controls. These results suggest that intra-articular injection of Bcl-xL-engineered MSCs is a potential non-invasive therapeutic method for effectively treating cartilage defects of the knee.

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Citations

Jun 26, 2014·BioMed Research International·Saliya Gurusinghe, Padraig Strappe
Jun 9, 2016·Stem Cells and Development·Janina Frisch, Magali Cucchiarini
Dec 6, 2018·Nature Reviews. Rheumatology·Magali Cucchiarini, Henning Madry
Dec 21, 2011·Molecular Biology Reports·Yiying QiWeiqi Yan
Mar 11, 2017·Journal of Orthopaedic Surgery and Research·Andy GoldbergRazi Zaidi

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