Enhanced triterpene and phytosterol biosynthesis in Panax ginseng overexpressing squalene synthase gene

Plant & Cell Physiology
Mi-Hyun LeeYong-Eui Choi

Abstract

Roots of Panax ginseng, one of the most famous medicinal plants, contain various phytosterols and bioactive triterpene saponins (ginsenosides). In P. ginseng, phytosterols and triterpenes share the common biosynthetic intermediate, squalene. Here, we investigate the regulatory role of Panax ginseng squalene synthase (PgSS1) on the biosynthesis of phytosterols and triterpene saponins. PgSS1 transcripts are expressed ubiquitously in the various plant tissues, but higher in shoot apex and root. The transcript levels of PgSS1 increased markedly in the adventitious roots during 12- to 96-h period after metyl jasmonate (MeJA) treatment; MeJA treatment induced the activation of the transcripts of squalene epoxidase (SE), beta-amyrin synthase (bAS), but not cycloartenol synthase (CAS). Unlike MeJA treatment, overexpression of PgSS1 in adventitious roots of transgenic P. ginseng was followed by the up-regulation of all the downstream genes tested, such as SE, bAS, and CAS. The enhanced activity of PgSS1 enzyme resulted in remarkable increase of phytosterols as well as ginsenoside contents. These results demonstrate that PgSS1 is a key regulatory enzyme not only for phytosterol but also for triterpene biosynthesis and overexpressing of P...Continue Reading

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Citations

Jun 26, 2012·Planta·Shuiqin WuJoe Chappell
Jan 27, 2011·Applied Biochemistry and Biotechnology·Ellen LambertDanny Geelen
Jan 23, 2013·Molecular Biotechnology·Shikha KalraKashmir Singh
Aug 3, 2011·Journal of Agricultural and Food Chemistry·Eleni NaziriMaria Z Tsimidou
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May 27, 2014·Applied Microbiology and Biotechnology·Hosakatte Niranjana MurthyKee-Yoeup Paek
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Feb 11, 2015·International Journal of Molecular Sciences·Xiasheng ZhengWeiwen Chen
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Dec 24, 2015·Protein Expression and Purification·Liangping ZhaLuqi Huang
Jan 15, 2013·Journal of Bioscience and Bioengineering·Abhinav GroverDurai Sundar
Feb 22, 2011·Phytochemistry·Jörg M AugustinSøren Bak

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