Enhanced welan gum production using a two-stage agitation speed control strategy in Alcaligenes sp. CGMCC2428

Bioprocess and Biosystems Engineering
Hui LiPing-Kai Ouyang

Abstract

Batch fermentative production of welan gum by Alcaligenes sp. CGMCC2428 was investigated under various oxygen supply conditions using regulating agitation speed. Based on a three kinetic parameters analysis that includes specific cell growth rate (μ), specific glucose consumption rate (q (s)), and specific welan formation rate (q (p)), a two-stage agitation speed control strategy was proposed to achieve high concentration, high yield, and high viscosity of welan. During the first 22 h, the agitation speed in 7.5 L fermenter was controlled at 800 rpm to maintain high μ for cell growth. The agitation was then reduced step-wise to 600 rpm to maintain a changing profile with stable dissolved oxygen levels and obtain high qp for high welan accumulation. Finally, the maximum concentration of welan was reached at 26.3 ± 0.89 g L(-1) with a yield of 0.53 ± 0.003 g g(-1) and the welan gum viscosity of 3.05 ± 0.10 Pa s, which increased by an average of 15.4, 15.2, and 20.1% over the best results controlled by constant agitation speeds.

References

Jan 15, 1994·Carbohydrate Research·R ChandrasekaranE J Lee
Oct 31, 1998·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·J CarlforsK Jörnving
Nov 7, 2000·Enzyme and Microbial Technology·F García-OchoaV E Santos
Aug 26, 1960·Biochimica Et Biophysica Acta·T C LAURENTA PIETRUSZKIEWICZ
May 29, 2008·Applied Microbiology and Biotechnology·Arsenio M FialhoIsabel Sá-Correia

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Citations

Sep 27, 2013·World Journal of Microbiology & Biotechnology·Zai-wei ManZheng-hong Xu
Feb 11, 2014·International Journal of Biological Macromolecules·Varinder KaurJ F Kennedy
Oct 30, 2016·International Journal of Biological Macromolecules·Abid HussainMohammad Zuber
Jul 8, 2016·International Journal of Toxicology·Monice M FiumeF Alan Andersen
Apr 4, 2017·Applied Biochemistry and Biotechnology·Panyu LiYongkui Zhang

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