Enhancement by conditioned medium of stretched calvarial bone cells of the osteoclast-like cell formation induced by parathyroid hormone in mouse bone marrow cultures

Archives of Oral Biology
S SomaT Takano-Yamamoto

Abstract

To investigate whether mechanical deformation of osteoblasts exerts an influence upon parathyroid hormone (PTH)-induced bone resorption, the effect of medium conditioned by a statistically stretched, osteoblast-enriched, bone cell population on PTH-induced osteoclast-like cell formation in mouse bone marrow cultures was examined. The conditioned medium of stretched cultures stimulated bone marrow cells to differentiate into tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cells in the presence of 10(-8) M PTH(1-34). The stimulatory effect of the conditioned medium was significant when the bone cells were stretched at 2 mm deflection in the 90% subconfluent state. Conditioned medium from cultures in the 50% subconfluent state failed to enhance osteoclast-like cell formation. PTH-induced, TRAP-positive multinuclear cells were decreased in number by the addition of stretch-conditioned medium in the postconfluent state. When 10(-6) M indomethacin was added to bone cell cultures during stretching, the resulting conditioned medium suppressed PTH-induced, TRAP-positive multinuclear cell formation. However, even if 10(-8) M prostaglandin E2 was added to the stretched bone cell cultures along with the indomethacin, the r...Continue Reading

References

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Citations

Dec 28, 1999·Journal of Biomechanics·T D Brown
May 11, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S SomaK Kurisu
Apr 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y AzumaS Jingushi
Dec 16, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Chi Hyun KimX Edward Guo
Oct 25, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Kenji HoshiTeruko Takano-Yamamoto

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