Enhancement of 2,4-dinitrotoluene biodegradation by Burkholderia sp. in sand bioreactors using bacterial hemoglobin technology

Biodegradation
Juho SoKrishna Pagilla

Abstract

Continuous flow sand column bioreactor experiments were conducted to investigate the effect of 2,4-dinitrotoluene (DNT) concentration (i.e. DNT loading rate) and influent dissolved oxygen (DO) concentration on aerobic biodegradation of DNT by wild type (DNT) and recombinant (YV1) Burkholderia sp., the latter containing plasmid pSC160 which carries the gene (vgb) encoding the hemoglobin (VHb) from the bacterium Vitreoscilla. The experiments were conducted in two continuous flow packed bed sand column bioreactors, one growing the wild type strain and the other growing YV1. Under oxygen-rich feed conditions (6.8 mg DO/L in the feed) with an influent DNT concentration of 99.6 mg/L (DNT loading rate approximately = 9.2 mg/m2/day), the effluent DNT concentration from the wild type bioreactor reached 0.7 mg DNT/L in 40 days whereas it was less than 0.2 mg DNT/L for the YV1 bioreactor in about 25 days. When influent DNT concentration was increased to 214 mg/L (DNT loading rate approximately = 20.3 mg/m2/day) while maintaining the same influent DO level of 6.8 mg/L, the effluent DNT concentration increased to about 5 mg/L for the wild type bioreactor whereas it was maintained at less than 0.2 mg/L for the YV1 bioreactor. Additionally, w...Continue Reading

Citations

Feb 26, 2013·Applied Microbiology and Biotechnology·Marina ArnaldosKrishna R Pagilla
Feb 11, 2012·Bioprocess and Biosystems Engineering·Tien-Tsai SuChih-Hung Wu
Jan 1, 2012·Computational and Structural Biotechnology Journal·Benjamin C StarkKrishna R Pagilla
Jan 13, 2015·Applied Microbiology and Biotechnology·Benjamin C StarkKanak L Dikshit
Sep 21, 2011·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Jan PacaEvguenii Kozliak
Aug 23, 2006·Critical Reviews in Biotechnology·Meltem Urgun-DemirtasKrishna Pagilla
Nov 9, 2005·Water Environment Research : a Research Publication of the Water Environment Federation·Meltem Urgun-DemirtasKrishna Pagilla

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