Enhancement of acetyl-CoA flux for photosynthetic chemical production by pyruvate dehydrogenase complex overexpression in Synechococcus elongatus PCC 7942.

Metabolic Engineering
Yasutaka HirokawaTaizo Hanai

Abstract

Genetic manipulation in cyanobacteria enables the direct production of valuable chemicals from carbon dioxide. However, there are still very few reports of the production of highly effective photosynthetic chemicals. Several synthetic metabolic pathways (e.g., isopropanol, acetone, isoprene, and fatty acids) have been constructed by branching from acetyl-CoA and malonyl-CoA, which are key intermediates for photosynthetic chemical production downstream of pyruvate decarboxylation. Recent reports of the absolute determination of cellular metabolites in Synechococcus elongatus PCC 7942 have shown that its acetyl-CoA levels corresponded to about one hundredth of the pyruvate levels. In short, one of the reasons for lower photosynthetic chemical production from acetyl-CoA and malonyl-CoA was the smaller flux to acetyl-CoA. Pyruvate decarboxylation is a primary pathway for acetyl-CoA synthesis from pyruvate and is mainly catalyzed by the pyruvate dehydrogenase complex (PDHc). In this study, we tried to enhance the flux toward acetyl-CoA from pyruvate by overexpressing PDH genes and, thus, catalyzing the conversion of pyruvate to acetyl-CoA via NADH generation. The overexpression of PDH genes cloned from S. elongatus PCC 7942 signific...Continue Reading

References

Jan 1, 1987·Methods in Enzymology·S S GoldenR Haselkorn
May 17, 2000·Methods in Enzymology·C R AnderssonS S Golden
Nov 17, 2009·Nature Biotechnology·Shota AtsumiJames C Liao
Aug 11, 2010·Journal of Bioscience and Bioengineering·Kentaro InokumaTaizo Hanai
Apr 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Xinyao LiuRoy Curtiss
May 17, 2011·Metabolic Engineering·Ethan I Lan, James C Liao
Jan 28, 2012·Journal of Bioscience and Bioengineering·Hiroko KatoAkihiko Kondo
Mar 27, 2012·Journal of Biotechnology·Iara M P Machado, Shota Atsumi
Jan 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·John W K OliverShota Atsumi
Jan 31, 2013·Nature Communications·Peng XuMattheos A G Koffas
Apr 13, 2013·Current Opinion in Biotechnology·Shuchi H Desai, Shota Atsumi
Jan 15, 2014·Metabolic Engineering·John W K OliverShota Atsumi
Jan 29, 2014·Journal of Biological Engineering·Daniel CamsundPeter Lindblad
May 7, 2014·The Journal of Biological Chemistry·Mulchand S PatelFrank Jordan
Oct 12, 2014·Current Opinion in Biotechnology·Philipp Savakis, Klaas J Hellingwerf
Dec 3, 2014·Journal of Bioscience and Bioengineering·Yasutaka HirokawaTaizo Hanai
Sep 11, 2016·Current Opinion in Chemical Biology·Neal J OliverShota Atsumi
Dec 1, 2017·Metabolic Engineering Communications·Rui MiaoPeter Lindblad

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Citations

Apr 29, 2020·Metabolites·Jason T KuEthan I Lan
Jan 26, 2021·Frontiers in Bioengineering and Biotechnology·Sheida StephensD Grant Allen
Aug 4, 2021·Nature Reviews. Microbiology·José Montaño LópezJosé L Avalos
Oct 26, 2021·Current Opinion in Biotechnology·Damini JaiswalPramod P Wangikar
Nov 16, 2021·Critical Reviews in Biotechnology·Yu-Lei JiaQi Li

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