PMID: 9548268Apr 21, 1998Paper

Enhancement of albumin production by hepatocyte growth factor in rat hepatocytes: distinction in mode of action from stimulation of DNA synthesis

Liver
M YamaokaK Fujiwara

Abstract

Hepatocyte growth factor (HGF) has been shown to enhance albumin production as well as stimulate DNA synthesis in hepatocytes. The mode of action of HGF in exerting both effects is to be elucidated. We previously observed that hepatocyte proliferation occurred in normal rats given recombinant human HGF (rhHGF) intravenously. When rats received rhHGF similarly, serum albumin levels were significantly increased compared to controls. In primary culture of rat hepatocytes, albumin concentration in culture medium was significantly increased by rhHGF added at 24 h of culture compared to controls, increasing with time of culture. This effect of rhHGF was dose-related. When actinomycin D was added to the medium, the albumin concentration was reduced in a dose-related manner, but its enhancement by rhHGF was maintained. Albumin mRNA levels were not increased by rhHGF. When rhHGF was added similarly to the medium, immunocytochemically positive hepatocytes for 5-bromo-2'-deoxyuridine (BrdU) incorporation appeared 30 h later. Of these labeled hepatocytes, 12.5% were concomitantly stained for albumin. In contrast, albumin-positive hepatocytes were seen in 77% of BrdU-non-labeled hepatocytes (p<0.01). We conclude that HGF may enhance albumin...Continue Reading

References

Jan 1, 1976·Methods in Cell Biology·P O Seglen
Jan 1, 1992·Journal of Cellular Physiology·J HernandezG K Michalopoulos
Mar 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M A RothschildS S Schreiber
Jun 1, 1995·The Journal of Cell Biology·R Zarnegar, G K Michalopoulos
Feb 4, 1995·Lancet·P Boros, C M Miller
Jun 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M I GuillénJ V Castell

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Citations

Feb 10, 2000·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·S EguchiT Kanematsu
Dec 12, 2013·Medical Molecular Morphology·Mineko ShibayamaShotaro Sakisaka
Feb 21, 2016·In Vitro Cellular & Developmental Biology. Animal·Tateki SumiiSusumu Kudo
Feb 13, 2001·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·H BarleJ Wernerman
Oct 10, 2002·Biochemical and Biophysical Research Communications·Tomoaki TomiyaKenji Fujiwara
Dec 20, 2003·Biochemical and Biophysical Research Communications·Tomoaki TomiyaKenji Fujiwara
Sep 1, 2004·Biochemical and Biophysical Research Communications·Tomoaki TomiyaKenji Fujiwara

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