Enhancement of Antioxidative and Intestinal Anti-inflammatory Activities of Glycated Milk Casein after Fermentation with Lactobacillus rhamnosus 4B15

Journal of Agricultural and Food Chemistry
Nam Su OhSae Hun Kim

Abstract

In this study, we investigated the glycoproteomics of glycated milk casein (GMC) and GMC fermented by Lactobacillus rhamnosus 4B15 (FGMC) and determined their biological implications. There was a significant increase in the antioxidative and anti-inflammatory activities of GMC with galactose, which were higher than those of GMC with glucose (GMC-glc). Furthermore, the fermentation of GMC by L. rhamnosus 4B15 synergistically enhanced the above activities compared to those of unfermented GMC. Especially, fermented GMC-glc (FGMC-glc) possessed remarkably improved reducing power and radical scavenging activities. Moreover, FGMC-glc ameliorated the inflammatory response and tight junction-related intestinal epithelial dysfunction. Additionally, hexose-derived glycation and modification sites in protein sequences of GMC were identified. In particular, glycosylation and sulfation of serine and threonine residues were observed, and distinct modification sites were detected after fermentation. Therefore, these results indicated that glycation-induced modification of casein and fermentation correlated strongly with the enhanced functional properties.

References

Jul 1, 1996·The Biochemical Journal·R M DanielH H Petach
Jan 27, 2000·Cell·T Hunter
Feb 27, 2001·Rapid Communications in Mass Spectrometry : RCM·M GalvaniP G Righetti
Jul 18, 2001·Die Nahrung·M A van Boekel
Oct 16, 2001·Journal of Agricultural and Food Chemistry·J Leclère, I Birlouez-Aragon
Feb 28, 2002·Annual Review of Immunology·Charles A Janeway, Ruslan Medzhitov
May 29, 2003·Current Pharmaceutical Design·H Korhonen, A Pihlanto
Feb 10, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Thomas Y MaHamid M Said
Apr 20, 2006·Critical Reviews in Food Science and Nutrition·Christine M OliverRoger A Stanley
Jul 16, 2008·Inflammatory Bowel Diseases·Michael A McGuckinGraham Radford-Smith
Apr 15, 2011·Critical Reviews in Food Science and Nutrition·Christine M Oliver
Mar 12, 2013·Gut·Mary Ellen SandersEmeran A Mayer
Nov 2, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Aline WitaicenisLuiz Claudio Di Stasi
Nov 20, 2013·Journal of Proteome Research·Florian BaumMonika Pischetsrieder
Dec 20, 2013·Current Nutrition and Food Science·R K Rao, G Samak
Jan 9, 2016·Journal of Proteomics·Sanja Milkovska-Stamenova, Ralf Hoffmann
Jan 14, 2016·Korean Journal for Food Science of Animal Resources·Minyu SongSejong Oh
Feb 9, 2016·International Journal of Biological Macromolecules·Jue TuYueqin Cai
Jul 2, 2016·International Journal of Cancer. Journal International Du Cancer·Hannah R WardillStephen T Sonis

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Citations

Dec 5, 2020·Journal of Dairy Science·Jae Yeon JoungSae Hun Kim
May 4, 2021·Comprehensive Reviews in Food Science and Food Safety·Jie GaoYaxin Sang

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