PMID: 12764105May 24, 2003Paper

Enhancement of associative long-term potentiation by activation of beta-adrenergic receptors at CA1 synapses in rat hippocampal slices

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Yi-Wen LinHsiu-Wen Yang

Abstract

The objective of this study was to evaluate the role of beta-adrenergic receptors in modulating associative long-term potentiation (LTP) induced at CA1 synapses. Two independent Schaffer collateral pathways were stimulated in hippocampal slices. The field EPSP (fEPSP) response evoked in one pathway (the weak pathway) was small, whereas a large response, usually 80-90% of the maximum, was evoked in the strong pathway. After recording of the baseline fEPSP evoked at 0.033 Hz, LTP of the weak pathway could be associatively induced by paired stimulation of the weak and strong pathways 100 times at 6 sec intervals, with stimulation of the weak pathway preceded 3-10 msec. However, pairing protocols with an interval between stimulation of the two pathways >10 msec resulted in no LTP. The induced LTP was NMDA receptor dependent, because 50 microm D,L-APV blocked its induction. Bath application of 1 microm isoproterenol enhanced LTP by increasing the window of the stimulation interval up to 15 msec but did not affect the magnitude of the LTP induced by pairing protocols with intervals <10 msec. Similar results were obtained when the experiments were repeated using whole-cell recording. These results suggest that activation of beta-adren...Continue Reading

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