Enhancement of avermectin and ivermectin production by overexpression of the maltose ATP-binding cassette transporter in Streptomyces avermitilis

Bioresource Technology
Meng LiJilun Li

Abstract

We investigated the function of maltose ABC transporter system encoded by malEFG-a and the effect of its overexpression on antibiotic production in Streptomyces avermitilis. A malEFG-a deletion mutant was unable to grow in a minimal medium with maltose as sole carbon source and produce avermectin. Maltose utilization and avermectin production were restored by introduction of a single copy of malEFG-a. RT-PCR analysis showed that the expression of malE-a was induced by maltose, and was strongly repressed by glucose. When multi-copy, integrative malEFG-a gene expression vectors were introduced into wild-type strain ATCC31267 and ivermectin-producer OI-31, antibiotic production increased by 2.6- to 3.3-fold and the time required for fermentation decreased by about 10%. The overexpression of malEFG-a improved the utilization rate of starch, and thereby enhanced avermectin production. Such an approach would be useful for the improvement of commercial antibiotic production using starch as the main carbon source in the fermentation process.

References

Mar 1, 1979·Antimicrobial Agents and Chemotherapy·R W BurgS Omura
Mar 1, 1979·Antimicrobial Agents and Chemotherapy·T W MillerI Putter
Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
Feb 1, 1991·Molecular Microbiology·E StrauchM J Bibb
Feb 1, 1988·Antimicrobial Agents and Chemotherapy·H IkedaS Omura
Aug 26, 1983·Science·W C CampbellT A Jacob
Sep 8, 1995·Cell·C F Higgins
Jun 26, 2001·Research in Microbiology·E Schneider
Feb 20, 2002·Chemical Reviews·Haruo Ikeda, Satoshi Omura
Dec 3, 2003·Parasitology Research·Isabel G Fernandez-de-MeraYolanda Fierro
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Amy L DavidsonJue Chen
Feb 24, 2009·Nature Reviews. Molecular Cell Biology·Douglas C ReesOded Lewinson

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Citations

Jun 30, 2011·Applied Microbiology and Biotechnology·Jingfan QiuZixin Deng
Feb 22, 2012·Journal of Industrial Microbiology & Biotechnology·Xiaojuan ZhangJilun Li
Apr 13, 2013·World Journal of Microbiology & Biotechnology·Xuejin ZhaoYuan Song
May 24, 2012·The Journal of Antibiotics·Lyudmila N LysenkovaMaria N Preobrazhenskaya
Dec 2, 2011·Microbes and Environments·Sylvain LeratCarole Beaulieu
Aug 12, 2014·Applied Microbiology and Biotechnology·Nguyen Huy ThuanJae Kyung Sohng
Dec 24, 2019·Current Protein & Peptide Science·Xin XieHui Jiang
Apr 23, 2020·Frontiers in Bioengineering and Biotechnology·Dongbo CaiShouwen Chen
May 2, 2016·Science China. Life Sciences·Jinsong ChenLixin Zhang
Oct 20, 2017·Current Opinion in Biotechnology·Qiang GaoLixin Zhang
Mar 23, 2021·Frontiers in Bioengineering and Biotechnology·Haijie XueChun Li
Jun 9, 2021·Current Opinion in Biotechnology·Shanshan LiLixin Zhang

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