Enhancement of Biodegradable Plastic-degrading Enzyme Production from Paraphoma-like Fungus, Strain B47-9

Journal of Oleo Science
Yuka Sameshima-YamashitaHiroko K Kitamoto

Abstract

To improve the productivity of Paraphoma-like fungal strain B47-9 for biodegradable plastic (BP)-degrading enzyme (PCLE), the optimal concentration of emulsified poly(butylene succinate-co-adipate) (PBSA) in the medium was determined. Emulsified PBSA was consumed as a sole carbon source and an inducer of PCLE production by strain B47-9. Among the various concentrations of emulsified PBSA [0.09-0.9% (w/v)] used in flask cultivation, 0.27% yielded the maximum enzyme activity within a short cultivation period. To evaluate the residual concentration of emulsified PBSA in culture, emulsified PBSA in aliquots of culture supernatant was digested in vitro, and the concentration of released monomerised succinic acid was determined. Regardless of the initial concentration of emulsified PBSA in medium, PCLE activity was detected after residual succinic acid decreased below 0.04 mg/mL in culture broth. Jarfermentation was performed at a 0.27% PBSA concentration. Among the various airflow rates tested, 1 LPM resulted in a PCLE production rate of 1.0 U/mL/day. The enzyme activity in the resulting culture filtrate (4.2 U/2 mL) was shown to degrade commercial BP films (1 × 1 cm, 20 µm thickness) within 8 hours.

References

Jun 22, 2005·Applied Microbiology and Biotechnology·Hiroshi MaedaTasuku Nakajima
Jun 30, 2011·Applied Microbiology and Biotechnology·Peter James BakerJin Kim Montclare
Dec 1, 2011·AMB Express·Hiroko K KitamotoSeiya Tsushima
Jan 13, 2012·Applied Biochemistry and Biotechnology·Denise Castro-OchoaAmelia Farrés
Jun 9, 2012·Applied Microbiology and Biotechnology·Yukiko ShinozakiHiroko K Kitamoto

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Citations

Jun 21, 2016·Journal of Oleo Science·Motoo KoitabashiHiroko Kitamoto
Nov 1, 2016·Genome Announcements·Yuka Sameshima-YamashitaHiroko Kitamoto
Nov 20, 2019·Biochimica Et Biophysica Acta. Proteins and Proteomics·Aneta K UrbanekMiguel Arroyo

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