Enhancement of biophysical activity of lung surfactant extracts and phospholipid-apoprotein mixtures by surfactant protein A

Chemistry and Physics of Lipids
A R VenkitaramanR H Notter

Abstract

The effects of surfactant protein (SP)-A on the dynamic surface tension lowering and resistance to inhibition of dispersions of calf lung surfactant extract (CLSE) and mixtures of synthetic phospholipids combined with SP-B,C hydrophobic apoproteins were studied at 37 degrees C and rapid cycling rate (20 cycles/min). Addition of SP-A to CLSE, which already contains SP-B and -C, gave a slight improvement in the time course of surface tension lowering on an oscillating bubble apparatus in the absence of inhibitory protein molecules such as albumin or hemoglobin. However, when these proteins were present at concentrations of 10-50 mg/ml, SP-A substantially improved the resistance of CLSE to their inhibitory effects. The beneficial effect of SP-A required the presence of Ca2+ ions, and disappeared when EDTA was substituted for this divalent cation in the subphase. The effect was also retained when SP-A was heated to 50 degrees C prior to addition to CLSE, but was abolished by heating SP-A to 99 degrees C. Additional studies showed that similar improvements in resistance to inhibition were found when SP-A was added to synthetic mixtures of dipalmitoyl phosphatidylcholine (DPPC):egg phosphatidylglycerol (PG) (80:20 by weight) reconsti...Continue Reading

Citations

Jan 1, 1994·Intensive Care Medicine·R Spragg
Apr 1, 1992·The International Journal of Biochemistry·T Cserháti, M Szögyi
Feb 6, 2004·The Journal of Thoracic and Cardiovascular Surgery·Andreas GüntherIvar Friedrich
Feb 14, 2002·Chemistry and Physics of Lipids·Robert H NotterB A Holm
Mar 6, 2004·Seminars in Neonatology : SN·Thierry Lacaze-Masmonteil
Oct 7, 2000·Respirology : Official Journal of the Asian Pacific Society of Respirology·I R DoyleT E Nicholas
Apr 30, 2005·Biology of the Neonate·A CalkovskaB Robertson
Jul 9, 1998·American Journal of Respiratory and Critical Care Medicine·Z Wang, R H Notter
May 6, 1999·American Journal of Respiratory and Critical Care Medicine·H William TaeuschJ A Clements
Aug 10, 2000·American Journal of Respiratory and Critical Care Medicine·K W LuJ A Clements
Jan 1, 1993·The American Review of Respiratory Disease·J F Lewis, A H Jobe
Dec 11, 1991·American Journal of Respiratory Cell and Molecular Biology·S HorowitzE R Cheng
Aug 19, 2006·American Journal of Respiratory Cell and Molecular Biology·Judy M Hickman-DavisRobert H Notter
Oct 13, 2000·Biochimica Et Biophysica Acta·E J Veldhuizen, H P Haagsman
Oct 5, 2001·Expert Opinion on Pharmacotherapy·D Willson
Apr 25, 2008·Biochimica Et Biophysica Acta·Yi Y ZuoFred Possmayer
Dec 7, 2000·Biophysical Journal·E J VeldhuizenH P Haagsman
Jul 18, 2008·Respiratory Physiology & Neurobiology·Sandra RugonyiStephen B Hall
Apr 8, 2006·Chemistry and Physics of Lipids·Alicia G Serrano, Jesús Pérez-Gil
Jan 5, 2006·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·S ParmigianiG Bevilacqua
Jul 10, 1999·Pediatric Research·B A HolmR H Notter
Jul 20, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·D L H PoelmaJ F van Iwaarden
Aug 18, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kaushik NagFred Possmayer
May 17, 2002·The Journal of Biological Chemistry·Nades PalaniyarFrancis X McCormack
Jun 19, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·K Rodriguez-CapoteF Possmayer
Feb 1, 1996·Pediatric Research·R BruniF J Walther
Jun 16, 1999·Journal of Applied Physiology·T KobayashiK Yamamoto
Aug 12, 1998·The American Journal of Physiology·M IkegamiA H Jobe
Nov 1, 1996·Thorax·M J KreschR S Thrall
Nov 7, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Neus LozanoRamon Pons
Jan 1, 1995·Life Sciences·L R McLean, J E Lewis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.