PMID: 7540748Mar 1, 1995Paper

Enhancement of Ca2+ channel currents in human neuroblastoma (SH-SY5Y) cells by phorbol esters with and without activation of protein kinase C

Pflügers Archiv : European journal of physiology
H L ReeveC Peers

Abstract

The effects of phorbol esters on Ca2+ channel currents in human neuroblastoma SH-SY5Y cells were studied using whole-cell patch-clamp recordings. Bath application of 12-O-tetradecanoylphorbol-13-acetate (TPA) or phorbol 12,13-dibutyrate (PDBu; 100 nM to 1 microM), known activators of protein kinase C (PKC), enhanced Ca2+ channel currents in a voltage-dependent manner similar to that of Bay K 8644. TPA also enhanced Ca2+ channel currents during cell dialysis with the PKC pseudosubstrate, PKC(19-36), and in cells which had been pre-incubated with 500 nM staurosporine, and which were exposed to staurosporine during recordings. Application of 4 alpha-phorbol-12,13-didecanoate (4 alpha-PDD; 100 nM), which does not activate PKC, caused current enhancement similar to the effects of TPA. However, intracellular dialysis of TPA was without effect on Ca2+ channel currents. Residual Ca2+ channel currents recorded after exposure to 1 microM omega-conotoxin GVIA were still enhanced by TPA, but in the presence of either Bay K 8644 (5 microM) or nifedipine (5 microM), TPA was without effect. When cells were pre-incubated for 10 min at 37 degrees C with 100 nM TPA, currents subsequently recorded in its absence were enhanced as compared to untre...Continue Reading

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Citations

Mar 5, 1999·Molecular Neurobiology·P F VaughanC Peers
Feb 12, 1998·European Journal of Pharmacology·R A PeterfreundJ S Fink
Dec 16, 1998·European Journal of Pharmacology·S LazarenoN J Birdsall
Apr 20, 2001·Brain Research. Molecular Brain Research·N J WebsterC Peers
Jan 13, 2009·Nature Neuroscience·Zhenjie ZhangMeyer B Jackson
May 3, 2005·Brain Research·Urban KarlssonJohannes J Krupp
Jan 21, 2012·Neurochemistry International·Vera CortezCláudia Cavadas
Jan 15, 2004·The Journal of Biological Chemistry·Sharon WeissNathan Dascal
Dec 14, 2001·The Journal of Biological Chemistry·Yakov BlumensteinNathan Dascal
Feb 6, 2002·The Journal of Biological Chemistry·Hiroyuki WatanabeBernd Nilius

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