Enhancement of calcification by osteoblasts cultured on hydroxyapatite surfaces with adsorbed inorganic polyphosphate

In Vitro Cellular & Developmental Biology. Animal
Kan KatoKazuhiro Tsuga

Abstract

Inorganic polyphosphate has been expected to accelerate bone regeneration. However, there are limited evidences to prove that polyphosphate adsorbed on the surface of a hydroxyapatite plate enhances calcification of cultured osteoblasts. In this study, we examined the effect of polyphosphate adsorbed onto the surface of a hydroxyapatite plate on the attachment, proliferation, differentiation, and calcification of osteoblasts. After hydroxyapatite plates were soaked in solutions of polyphosphate, the plate surfaces were analyzed by scanning electron microscopy and toluidine blue staining to confirm adsorption of polyphosphate. The hydroxyapatite plates were further subjected to the measurements of surface roughness, water contact angle, and the binding capacity of calcium ions. Cell culture experiments were carried out using MC3T3-E1 pre-osteoblastic cells. It was found that soaking a hydroxyapatite plate in a polyphosphate solution gave rise to an increase in surface roughness and reduction in water contact angle in a concentration-dependent manner, suggesting the adsorption of polyphosphate onto the surface of a hydroxyapatite plate. It was further observed that surface-adsorbed polyphosphate exhibited an inhibitory effect on ...Continue Reading

References

Jan 1, 1977·Caries Research·J Arends, W L Jongebloed
Jun 4, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G LeyhausenH C Schröder
Aug 17, 1999·Progress in Molecular and Subcellular Biology·H C SchröderW E Müller
Dec 30, 1999·Biomaterials·D A Puleo, A Nanci
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·G R BeckE Moran
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 13, 2003·The Journal of Biological Chemistry·Toshikazu ShibaMinako Ohshiro
Jul 24, 2004·Journal of Dental Research·Y KawazoeT Kohgo
Jul 11, 2006·Journal of Biomedical Materials Research. Part a·Miho NakamuraKimihiro Yamashita
Sep 28, 2007·Journal of Materials Science. Materials in Medicine·R W N Nilen, P W Richter

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Citations

Nov 16, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Erik A B HughesLiam M Grover
Jul 28, 2019·BioMed Research International·Yan WangZhiliang Guo

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