PMID: 11342033May 9, 2001Paper

Enhancement of chymotrypsin-inhibitor/substrate interactions by 3 M NaCl

Biochimica Et Biophysica Acta
O WesołowskaJ Otlewski

Abstract

A series of 16 bovine pancreatic trypsin inhibitor variants mutated at the P(1) position of the binding loop and seven tetrapeptide p-nitroanilide (pNa) substrates of the general formula: suc-Ala-Ala-Pro-Aaa-pNa (where Aaa denotes either: Phe, Arg, Lys, Leu, Met, Nva, Nle) were used to investigate the influence of high salt concentration on the activity of bovine chymotrypsin. The increase of the association constant (K(a)) and the specificity index (k(cat)/K(m)) in the presence of 3 M NaCl highly depends on the chemical nature of the residue at the P(1) position. The highest increase was observed for inhibitors/substrates containing the basic side chains at this site. Surprisingly, for the remaining 13 residues the observed salt effect is not correlated with any side chain properties. In particular, there is a lack of correlation between the accessible non-polar surface area and the magnitude of the salt effect. It suggests that salt-induced increase of the K(a) and k(cat)/K(m) values is not caused by the enhancement of the hydrophobic interactions in chymotrypsin-inhibitor/substrate complex. Moreover, the increase of the K(a) and k(cat)/K(m) values occurs only in the presence of Na(+) ions, while K(+) and Li(+) ions do not ch...Continue Reading

References

Dec 29, 1967·Biochemical and Biophysical Research Communications·N AbramowitzA Berger
Apr 20, 1967·Biochemical and Biophysical Research Communications·I Schechter, A Berger
Oct 1, 1994·Protein Science : a Publication of the Protein Society·J P Staley, P S Kim
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·K D Collins
Sep 22, 1995·The Journal of Biological Chemistry·E Di CeraA Tulinsky
Jan 11, 1994·Biochemistry·M RheinneckerA R Fersht
Feb 21, 1997·Journal of Molecular Biology·W LuM Laskowski
Jul 24, 1998·Biochimica Et Biophysica Acta·J PolanowskaJ Otlewski
Nov 23, 1995·Nature·R Leberman, A K Soper

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Citations

Mar 14, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·A Nolan WilsonAnthony Guiseppi-Elie
Dec 23, 2009·Biochimica Et Biophysica Acta·Zhen YangPeter J Halling
Jul 5, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Monika ZelazkoAntoni Polanowski
Apr 26, 2006·Biochimica Et Biophysica Acta·Jolanta KowalskaTadeusz Wilusz

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