Enhancement of cytokine production by macrophages stimulated with (1-->3)-beta-D-glucan, grifolan (GRN), isolated from Grifola frondosa

Biological & Pharmaceutical Bulletin
Y AdachiT Yadomae

Abstract

The ability of grifolan (GRN), a purified fungal (1-->3)-beta-D-glucan, to induce various cytokines from macrophages was examined in vitro. Interleukin-6 (IL-6) activity in supernatants from the culture of macrophage cell line, RAW264.7 was dependent on increasing doses of GRN. The level of IL-6 induced with 500 micrograms/ml of GRN was comparable to that induced with lipopolysaccharide (LPS) 10 micrograms/ml. Enhancement of the mRNA level of IL-6 by treatment with GRN was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The effect of GRN on production of IL-6 was also observed using peritoneal macrophages from C3H/HeJ mice which did not respond to endotoxins. This data suggested that the ability of GRN to activate IL-6 production of macrophages is not due to contamination of endotoxins in the preparation. Enhanced production of cytokine by GRN was observed not only with IL-6, but also with interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF alpha). In the production of TNF alpha, GRN was more effective than LPS used in this study. Other soluble or gel-forming(1-->3)-beta-D-glucans from various sources did not enhance the production of such cytokines although they are structurally similar to GRN. The ...Continue Reading

Citations

Mar 26, 2014·International Journal of Molecular Sciences·Fa-Yan MengXue-Hua Li
Nov 13, 2009·Nutrition Reviews·Otakar RopTunde Jurikova
Apr 28, 2001·Mediators of Inflammation·B FogelmarkR Rylander
Oct 2, 2001·Mediators of Inflammation·J ThornR Rylander
Mar 17, 2005·Mediators of Inflammation·L Beijer, R Rylander
Jul 21, 2005·Mediators of Inflammation·Cristina LullHuub F J Savelkoul
Jul 20, 2010·Journal of Dental Research·C C TeixeiraM Alikhani
Mar 18, 2008·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Vaclav Vetvicka, Jana Vetvickova
Feb 24, 2005·Applied Microbiology and Biotechnology·Ben-Zion ZaidmanSolomon P Wasser
Feb 21, 2004·International Immunopharmacology·Hong LinSusanna Cunningham Rundles
Jul 24, 2013·Journal of Medicinal Food·Eliana Noelia AlonsoGabriela Andrea Balogh
Sep 10, 2008·Journal of Ethnopharmacology·Mei-Chun KuoMing-Jiuan Wu
Aug 9, 2015·Applied Microbiology and Biotechnology·Nicole DalonsoRegina Maria Miranda Gern
Jul 19, 2015·International Journal of Biological Macromolecules·Mayra A D SalehMarcos L P Tse
May 4, 2007·Antiviral Research·Chang-Qing GuZhi-Qiang Shen
Dec 26, 2009·Veterinary Immunology and Immunopathology·Eva SonckEric Cox
Dec 3, 2014·Developmental and Comparative Immunology·Kim BaertEric Cox
Sep 11, 2008·Scandinavian Journal of Immunology·G HetlandB Grinde
Jul 18, 2001·Biological & Pharmaceutical Bulletin·Y SuzukiT Yadomae
Jul 4, 1998·Scandinavian Journal of Immunology·G HetlandH G Wiker
Aug 24, 2004·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Toshie HaradaNaohito Ohno
Jun 17, 2003·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Y NakagawaY Haishima
Aug 24, 1999·European Journal of Immunology·G H WanR H Lin
Mar 21, 2000·American Journal of Industrial Medicine·J DouwesD Heederik
May 18, 2006·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Toshie HaradaNaohito Ohno
Feb 13, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Toshie HaradaNaohito Ohno

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